为了能有效地杀除共培养后残余的农杆菌和筛选转化体,测定了青菜对5种抗生素的敏感性。
For effective elimination of Agrobacterium and screen of transformed cells after co-cultivation, sensitivity of cabbage cultured in vitro to 5 antibiotics was testes.
共培养培养基中添加乙酰丁香酮可提高转化频率。
It was beneficial to add acetosyringone into cocultivation medium for improving transformation frequency.
从共培养到转化植株的获得只需6—7周。
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