...扫描共焦显微镜观察样品在不同的制备方式、激发波长、激发功率等条件下的SHG和双光子激发荧光(two-photon excited fluorescence, TPEF)的变化规律,实验还对鼠尾、脂肪的SHG和TPEF成像进行了比较。
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介绍采用双光子激发荧光方法进行单分子探测的原理和自行研制的实验装置，激发光聚焦和荧光收集采用共焦方式。
We report here briefly the principle and the device of our single-molecule detection spectrometer based on two-photon excited fluorescence.
研究了不同波长的双光子激发对成像效果的影响，优化了荧光激发与探测光路，开发并完善了三维图像的恢复重建和数据分析软件。
Studied on wavelength impacts on multi-photon excitation and optimized and fixed the optical design, compiled softwares for 3D construction and data analysis.
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