...培养 ; 低温预处理 ; 小孢子 ; 发育途径 [gap=1171]Key words: Rose roxburghii Tratt cv.; anther culture; cold pretreatment; microspore; developmental pathway ...
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Low temperature pretreatment favored the maintaining of microspore activity, but the effect on the microspore dedifferentiation was not obvious.5.
低温预处理有利于保持小孢子的活力,但对小孢子的脱分化作用不明显。
参考来源 - 辣椒花药小孢子培养及其胚状体发生机理研究The callus induction rate increased with 4℃cold pretreatment 3 days or 15℃low temperature culture for 3 days.
花药经4℃低温预处理3天或培养初期15℃低温预培养3天可以提高愈伤组织诱导率。
参考来源 - 苜蓿花药培养预处理方法和培养基的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
低温预处理有利于保持小孢子的活力；
The pretreatment with low temperature was beneficial to maintaining microspore vitality.
适当的低温预处理可以不同程度提高诱导率；
The proper low-temperature pretreatment can improve the initiation rate;
低温预处理可以诱导对致死浓度h2o2的抗性。
Pretreatment of low temperature could induce resistance to lethal concentration of H2O2.
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