目的:建立一个灵敏、准确、特异、试剂稳定的乙醇氧化酶法用于测定血清微量乙醇浓度。
Objective:To establish a sensitive, accurate, specific method to determine a small quantity of alcohol in serum with stable reagents by catalysis of alcoholic oxidase.
方法:利用乙醇氧化酶与过氧化物酶偶联,通过优化反应体系及自动生化分析仪分析参数,采用两点速率法对乙醇进行分析。
Methods: to analyze alcohol by two points rate method after optimizing reaction system and the assay parameter of automatic analyzer using alcoholic oxidase coupling peroxydase.
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