目的：系统的研究小鼠胚胎干细胞的提取、体外培养扩增和向成骨细胞定向诱导分化。
Objective: research systematically into the isolation, culture and direct differentiation to osteoblast of embryonic stem cells.
细胞水平上淫羊藿乙酸乙酯萃取部位可以促进小鼠骨髓基质细胞向成骨细胞分化而不抑制其向脂肪细胞分化。
On cellular level, the fraction of ethyl acetate could promote osteogenic differentiation and showed no inhibition on adipogenic differentiation of MSCs.
方法：(1)建立与改良小鼠成骨细胞的原代培养方法，并通过碱性磷酸酶和钙结节染色方法进行细胞来源鉴定。
Methods: (1) We built and improved the method of osteoblasts primary culture in vitro in mice, and identified the origination of osteoblasts by AKP and calcium nodes dyeing.
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