方法：(1)建立与改良小鼠成骨细胞的原代培养方法，并通过碱性磷酸酶和钙结节染色方法进行细胞来源鉴定。
Methods: (1) We built and improved the method of osteoblasts primary culture in vitro in mice, and identified the origination of osteoblasts by AKP and calcium nodes dyeing.
为了更好的了解胰岛素对于骨骼的影响，两个研究小组对实验小鼠进行了改造，使得它们的成骨细胞中不再有胰岛素受体。
To better comprehend insulin's effects on bone, both research teams engineered mice so that their osteoblasts no longer displayed insulin receptors.
目的：系统的研究小鼠胚胎干细胞的提取、体外培养扩增和向成骨细胞定向诱导分化。
Objective: research systematically into the isolation, culture and direct differentiation to osteoblast of embryonic stem cells.
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