Objective To optimize the transfection rate in COS 7 cell line with electroporation method.
目的以电穿孔法转染COS 7细胞系以得到较高的转染率。
While transformation efficiency was influenced by electric field intensity and pulse time when electroporation method was applied.
在进行电击法转化农杆菌时，电场强度和脉冲时间都对农杆菌的转化效率有影响。
The direct gene transfer systems involved the chemical method (PEG), electroporation, microinjection, particle bombardment, pollen-mediated gene transfer and other systems using various vectors.
阐述了化学导入法(PEG)、电穿孔法、注射法，粒子轰击法、花粉管萌导法及使用其它载体系统的成功实例。
应用推荐