Method: Using plant single cell plate culture technique.

方法：利用植物细胞平板技术培养。

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The results indicated that for culture of P. domesticum on agar plate, elution by sand washing method (140-mesh fine sand was used to replace glass beads in conventional method) had good efficacy.

结果表明，对琼脂平板上砖火丝菌培养物，采用沙洗法（以140目细沙代替常规法中的玻璃珠）洗脱，效果较好。

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Method: Based on enrichment culture, blood plate separation, the flask culture and exclusive circle of methods such as oil.

方法：富集培养、血平板分离、摇瓶培养和排油圈测定。

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