Method using weight 20-30g Kunming mouse as provider. establishan improved collagen perfusion technique to isolate the mousehepatocyte . then cryopreserve the isolated mouse hepatocyte with differentcryopreserved solutions,. with usual cryopreserved protocol in nitrogen liquid.
方法用体重20-30克左右的昆明小鼠为肝细胞供体，用改良的胶原酶灌注法分离肝细胞，将分离好的肝细胞分别以不同的冻存液进行逐级降温缓慢冻存，置液氮中。
参考来源 - 活体肝细胞分离冻存及其活性研究·2,447,543篇论文数据，部分数据来源于NoteExpress
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