DH5a containing the helper plasmid T i. The construction of the binary expression vector was correct, which was checked by PCR of the HW055 gene and the triparnetal mating Agro bacterium tumefactions was selected by culture medium containing many antibiotics.
通过Ti质粒共同组成双元表达载体系统。
参考来源 - 密码子优化的O型口蹄疫病毒VPI基因转化饲草玉米的高效表达载体构建·2,447,543篇论文数据，部分数据来源于NoteExpress
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