The use of temperature and acid concentration to increase the rate of sulfonation while controlling the side reaction of sulfone bridging minimizes reaction time while maximizing bead quality.
在控制砜桥副反应的同时，使用能够提高磺化速率的温度和酸浓度，使珠粒质量最佳化的同时降低了反应时间。
This part includes preparation of total protein samples from the culture cells, on-bead immuno-binding reaction and wash step, and choice of sampling.
包括细胞总蛋白样品的制备、微珠上抗原-抗体 反应及微珠洗涤条件、进样方式的 选择与改进。
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