Conclusions a clone of murine GAD65 is successfully constructed. It is a basis step for further construction of expression plasmid that expresses GAD65 and for immunologic intervention of T1DM.

结论成功的克隆了小鼠gad65基因，为构建GAD65基因疫苗以及糖尿病的免疫干预治疗打下基础。

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A system step, however, is not a good basis for delineating a use case transaction, for it depends on the granularity of the description of a flow how many steps you count.

但是，一个系统步骤并不是描述用例事务的一个较好的基础，因为它取决于对您计算的多少步骤的描述。

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Usually one step in the cycle serves as the basis for the next step, and the output of a former step is the input for the current one.

通常循环中的一个步骤被看作是下一个步骤的基础，上一个步骤的输出是当前步骤的输入。

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But the solution to that problem is to eliminate the step up in basis, not to introduce the Buffett Rule.

FORBES: The Arbitrariness of the Buffett Rule

Another important benefit of the NUA strategy is that for any shares not liquidated after distribution, a step up in basis is available at death.

FORBES: Tap Into Employer Stock Without Paying Taxes (For Now)

If grandparents sell appreciated stocks to raise that cash, they have to pay gain taxes and lose the opportunity to step up the basis in these stocks at their deaths.

FORBES: Magazine Article