Its equipment resources are extensive and include cell and tissue culture, fluorescence microscopy, recombinant DNA cloning and sequencing, recombinant protein purification, protein sequencing, peptide synthesis, mass spectroscopy, and advanced computers.
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表达产物用亲和层析一步法进行纯化,然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
介绍了近年来重组蛋白质的常用表达系统以及各种分离纯化技术等研究和应用的进展情况。
The study and application were introduced on the expression system in common use and separation and purification technique of recombinant proteins in recent years.
纯化的重组蛋白纯度约为93%。
The purified recombinant protein reached a purity of about 93%.
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