重组质粒的转化及筛选；
在植物基因工程中，农杆菌质粒介导的遗传转化是比较完善与有效的基因转移方法，但在单子叶植物上应用较少。
The gene transformation by agrobacterium'plasmid is a perfect and effective method of transferring gene in the gene engineering, however, there is a little applied on the monocotyledons plants.
来自受体菌自身的同源质粒，因克服了宿主的限制一修饰性，可以极显著地提高电转化效率。
A significant increase of transformation efficiency was revcaled with the homologous plasmid isolated from recipient itself since the effects of host restriction and modification werc avoided.
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