目的构建狂犬病病毒糖蛋白基因的真核细胞表达载体，并在COS - 7细胞中表达。
Objective To construct a recombinant plasmid for expression of rabies virus glycoprotein in COS-7 cells.
蛋白质磷酸化和去磷酸化作为原核和真核细胞表达调控的关键环节，了解其对功能的影响可以深入理解生命系统在分子水平的调控状况。
As the key point of expression modulation in prokaryotic and eukaryotic cells, the protein phosphorylation and dephosphorylation may help reveal the status of the life system at the molecular level.
提示，表达的H5蛋白为可控细胞膜孔形成蛋白，能用作真核细胞内玻璃态保存的渗透剂。
The results indicated that the expressed H5 protein is the controllable pore-forming protein and thus usable for intracellular vitrification preservation studies of mammalian cells.
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