这一概念是基于UNIX运行级别的概念：系统以级别1启动，然后单调递增，直到达到目标启动级别。

These are based on the concept of UNIX run levels; the system starts at level one, and then monotonically increments until it hits the target start level.

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一旦新目标自动启动，你才会开始下一个目标。

Once that new goal is on autopilot, only then do you start on your next goal.

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请记得在运行此步骤前启动目标应用服务器。

Remember to start the target application server before running this step.

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When this bound receptor interacts with DNA it could, for example, turn on expression of a target gene.

该受体与DNA结合后能够,启动目标基因的表达

耶鲁公开课 - 生物医学工程探索课程节选

What we're going to do is take this plasmid, with its promoter gene construct in it and engineer it, to take out the lacZ gene, leaving in the promoter and putting in the protein that we want - the gene for the protein that we want.

我们要做的就是提取包含,它自己的启动子基因的质粒,并对其进行一些修改,剔除lacZ基因,留下启动子,把我们的目标蛋白植入--,我们生产目标蛋白的基因

耶鲁公开课 - 生物医学工程探索课程节选