方法提取肿瘤组织总rna，用反转录聚合酶链式反应(RT - pcr)法进行检测。

Methods Total RNAs of tumor tissues were extracted and were detected by RT-PCR.

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干预：40只大鼠，于伤后不同时间取水肿区脑组织及正常脑组织：提取组织总rna：用共扩增反转录定量pcr方法测定MMP - 9基因表达水平。

INTERVENTIONS: Tissues of edema area and normal brain of 40 rats were collected in different time after damage: total RNA were abstracted and MMP-9 gene expression was tested by PCR means.

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用随机引物反转录，通过TC-RT-PCR检测ACLSV和ASGV复合感染的梨样品也均获得了目标片段。

A TC-RT-PCR method basing on reverse transcription with random primer facilitated the detection for those samples mixed infected by ASGV and ACLSV.

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It turns out that we can do that now because we have an enzyme called reverse transcriptase, which is able to take single stranded messenger RNA and make DNA out of it.

我们现在已经可以做到这一点了,因为我们有了反转录酶,反转录酶可以用,单链的mRNA创造出DNA

耶鲁公开课 - 生物医学工程探索课程节选

Reverse transcriptase is a naturally occurring protein, it has a biological function in HIV, but we can use it for a technical logical function here by going backwards on the biological path from messenger RNA to DNA.

反转录酶是在自然环境中存在的蛋白质,它在HIV中发挥着生物功能,但在这里它可以发挥一个技术功能,让它用信使RNA反转录出DNA

耶鲁公开课 - 生物医学工程探索课程节选