用8%非变性聚丙烯酰胺凝胶电泳将酶切产物分离，并用银染法显色。

The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.

youdao

用酶切和PCR的方法对重组质粒进行鉴定。

Used restrict enzyme and PCR to verify the reconstructed plasmid.

youdao

目的建立尿中性肽内切酶(NEP)检测的ELISA法，明确用ELISA法检测尿nep在诊断肾小管损伤中的意义。

Objective to set up an ELISA method to measure urinary neutral endopeptidase (NEP) and determine its clinical meaning in diagnosing renal tubular injury.

youdao

If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

耶鲁公开课 - 生物医学工程探索课程节选

This gives you a biological mechanism for cutting, using restriction enzymes, and then you denature so that it falls apart, and then you renature so that it comes back together.

这是一种切割DNA的生物机制,用限制性内切酶,改变DNA的性质让它打开,然后让它合起来恢复它的性质

耶鲁公开课 - 生物医学工程探索课程节选

Well the first step would be to cut open the plasmid with a particular restriction enzyme, and then what if I take that same restriction enzyme and I cut up the DNA that I'm interested in.

第一步是用某种限制性内切酶把质粒切开,然后用同一种限制性内切酶,切出我想要的DNA

耶鲁公开课 - 生物医学工程探索课程节选