...亡 C-7721细胞48h后,免疫荧光染色观察细胞凋亡情况:琼脂糖凝胶电泳观察凋亡细胞特征性DNA条带:流式细胞仪法(Flow cytometry,FCM)检测细胞凋亡和细胞周期:荧光定量PCR检测Fas和Caspase-3基因mRNA的表达水平;并比较阻...
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...7721细胞48h后,免疫荧光染色观察细胞凋亡情况:琼脂糖凝胶电泳观察凋亡细胞特征性DNA条带:流式细胞仪法(Flow cytometry,FCM)检测细胞凋亡和细胞周期:荧光定量PCR检测Fas和Caspase-3基因mRNA的表达水平;并比较阻...
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流式细胞仪分析法 Flow cytometer analytic method
方法应用流式细胞仪法检测30例透析及未透析患者外周血t细胞表面的CD 3、及其亚群cd4及CD 8抗原表达。
Methods Expression of CD3, CD4 and CD8 antigen on peripheral blood t cells from 30 cases of hemodialysis and non-hemodialysis patients was evaluated by flow cytometry.
方法:应用流式细胞仪法对328例AS患者1、113例腰背腿疼患者和40例健康体检人员进行了HLA-B27抗原检测。
Methods HLA-B27 detection was made in 328 patients with AS, 1113 patients with waist-back-leg ache and 40 healthy individuals.
结果形态学变化、流式细胞仪分析和TUNEL法结果均证实含鲜壁虎血清在体外可诱导C6胶质瘤细胞凋亡;
Results The C6 glioma cell apoptosis induction of GFP contained serum in vitro could be confirmed by morphological examination, flow cytometry analysis and TUNEL method.
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