引物的设计很关键，特异的引物序列设计参照文献。
Primer design is the key to our success, the primer sequences have designed specifically according to the reference.
结论从引物设计的过程中可以看到一种趋势-后期引物设计的成功率远远高于早期。
Conclusions There is a trend that can be recognized in the process of designing primer. Namely, the forepart is far more than the anaphase in the ratio of success to design primer.
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