GST_医学杂志_健康那些事儿K210.COM Keywords】 fusion protein； soluble expression； purification [gap=414]关键词】 融合蛋白；可溶表达；纯化
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Intracellular soluble expression of recombinant CypA was achieved by controlling temperature and rotaing speed.
通过培养温度与摇床转速的控制实现了重组CypA的胞内可溶表达。
参考来源 - 亲环蛋白A的制备、表征及协助蛋白质重折叠初步研究·2,447,543篇论文数据，部分数据来源于NoteExpress
拟获得在大肠杆菌中能高效可溶表达的转铁蛋白受体单链抗体与链亲和素（SA）的重组融合蛋白。
To obtain the anti-transferrin receptor scFv and streptavidin(SA) recombinant fusion proteins which were high-efficiently and solubly expressed in E.
结果：融合蛋白以可溶性形式存在于细菌裂解液的上清中，其表达量为菌体总蛋白量的7%。
RESULTS: Fusion protein existed in supernatant of the bacteria lysate and its expression level was about 7% of the total bacteria protein.
第一部分阐述了人胰岛素受体配基结合结构域L1和L2的可溶性表达并鉴定了它们的配基结合能力。
The first part of the thesis describes the expression and characterization of the human insulin receptor's ligand-binding domains L1 and L2.
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