方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.
方法：在表达及分离纯化VEGF的基础上，成功地制备了VEGF抗体，以夹心法测定vegf。
Methods: Based on isolation, purification and expression of VEGF, successfully prepared anti VEGF antibody and developed a sandwich radioimmunoassay for VEGF.
纯化的抗体不能直接用于其它已知抗原的检测，它是从天然兔血浆中分离出来的，通过蛋白A亲和色谱法制备。
The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography.
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