目的用亲和纯化法纯化嗜水气单胞菌气溶素，从单因子水平分析嗜水气单胞菌的溶血特性。
Methods:The proteins which interacted with gankyrin were screened and analyzed by affinity purification technology and mass spectrometry.
多克隆抗体通过用DYKDDDDK合成肽免疫动物制备。抗体用蛋白a和多肽亲和色谱法纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein a and peptide affinity chromatography.
结论用尼龙滤膜zbm改进的膜亲和层析法，可用于纯化腹水中的单克隆抗体，且简便、省时、有效。
Conclusion The modified MAC with ZBM is a much easier, time-saving and effective method for affinity purification of antibodies in ascites.
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