然后，我用另外10分钟的梯度把最终的条件重新平衡到初始的条件，接着保持8分钟等度洗脱。

Then, I use another 10-min gradient to reequilibrate from the final conditions back to the initial conditions, followed by 8 min of isocratic hold.

youdao

这个重新平衡由以初始的比例通过柱子的溶剂体积来决定，对于你的方法它是8分钟。

The reequilibration depends upon the volume of solvent passing through the column at the initial conditions, which is only 8 mL in your case.

youdao