聚合酶链反应-限制性片段长度多态性法;
Polymerase chain reaction restriction fragment length polymorphism (PCR RFLP);
运用限制性片段长度多态性分析对PCR扩增产物进行鉴定。
PCR product was confirmed by restriction fragment length polymorphism analysis.
用S基因聚合酶链反应-限制性片段长度多态性确定HBV基因型。
HBV genotypes were determined by RFLP based on S-gene PCR products.
方法应用聚合酶链反应限制性片段长度多态性技术检测296例两个基因多态位点的等位基因、基因型。
MethodGenotypes and alleles of polymorphisms of both genes were determined with polymerase chain reactionrestriction fragment length polymorphism assay (PCRRFLP) of 296 subjects in Han Chinese.
方法对110名重庆汉族个体,采用降落式PCR和限制性片段长度多态性法(RFLP)进行基因型分析。
Methods Single round Touchdown PCR (TD PCR) and restriction fragment length polymorphism (RFLP) method were used for genotyping in 110 people from the Han population in Chongqing.
采用多聚酶链反应-限制性片段长度多态性法(PCR - RFLP)分析MGP和ALAD基因的多态性。
The polymorphisms of MGP gene and ALAD gene were analyzed by the methods of PCR-RFLP.
方法应用多聚酶链反应技术及限制性片段长度多态现象对46例食管癌APC和MCC基因的LOH进行了分析。
Methods LOH at APC and MCC genetic loci in 46 specimens resected from esophageal neoplasm was studied with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
本文综述了DNA序列分析、P CR限制性片段长度多态性技术和环介导等温扩增技术在带绦虫虫种鉴别中的应用。
This paper summarizes the application of molecular biological techniques in the identification of Taenia, such as analysis of DNA sequence, PCR-RFLP and LAMP.
综述了白色念珠菌感染的分子生物学诊断研究进展,包括核型分析、限制性片段长度多态性分析、特异的DNA探针及PCR等方法。
The applications of karyotyping and restriction fragment length polymorphism (RFLP) analyses, specific DNA probes and PCR method to the diagnosis of Candida albicans infections are reviewed.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBVDNA阳性的上海籍HBV感染者的基因型。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
方法:选择符合入选标准的高血压患者336例,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法,进行ACE2基因分型。
Methods:336 hypertension patients were recruited in this trail. The distribution of ACE2 gene A9570G polymorphism was analyzed by PCR-RFLP in all participants.
方法应用聚合酶链反应限制性片段长度多态性分析方法(PCR -RFLP)对40例口腔鳞癌组织中apc基因的杂合缺失(LOH)进行检测。
Methods We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to examine 40 oral squamous cell carcinomas for loss of heterozygosity (LOH) at APC.
酚-氯仿法从外周血中提取基因组dna,聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
方法在140名汉族健康人的外周血中,应用聚合酶链反应(PCR)限制性片段长度多态性分析(RFLP)及多重PCR技术,进行NAT1等位基因分型研究。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
采用聚合酶链式反应(PCR)技术对来自北京等地区的173份PCV2阳性样本的全长ORF2基因进行了扩增,并对扩增产物进行了限制性片段长度多态性(RFLP)分析。
Whole ORF2 genes of 173 positive clinical PCV2 samples from Beijing and other areas were amplified by PCR and followed by restriction fragment length polymorphism (RFLP) analysis.
采用病例对照研究的方法,用聚合酶链反应(PCR)和限制性片段长度多态性分析(RFLP)研究TS患者与健康对照间DRD2P、COMT基因的基因型频率有无统计学差异。
DRD2P and COMT genotyping were carried out using PCR followed by RFLP and statistical analysis of genotype frequencies between ts patients and healthy controls was performed using SPSS programme.
方法应用聚合酶链反应( PCR)、DNA测序和限制性片段长度多态性(RFLP)等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
限制性酶切片段长度多态性(RFLP)作为共显性的遗传标记,已广泛应用于遗传病的产前诊断。
As a codominant genetic marker, restriction fragment length polymorphisms, referred as RFLPs, has been widely Applied to the prenatal diagnosis of some genetic diseases.
方法:采用PCR技术和限制性内切酶片段长度多态性(RFLP)的方法,检测中国北方汉族168个核心家系的基因型。
Methods A PCR-based RFLP procedure was employed to detect the genotypes of 168 family trios of Han descent population in the North of China.
方法:运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR -RFLP)检测MTHFR的677位点多态性。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
方法:运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR -RFLP)检测MTHFR的677位点多态性。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
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