• 重组酵母测试了11种原托普生二体外雌激素活性

    The vitro estrogenic activities of 11 protopanaxadiol type saponins were tested by a recombinant yeast.

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  • 研究了重组酵母测评系统中,重组酵母细胞条件优化问题。

    In this paper, the optimal condition of the yeast cell breaking was studied and finally confirmed through many experiments.

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  • 因此,研究人员尝试各种方法构建重组酵母提高木糖发酵能力

    Hence attempts have been made to construct various recombinant yeasts to improve the ability of xylose fermentation.

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  • 研究表明,纤维素半纤维素水解产生的葡萄糖木糖重组酵母S

    The results showed that glucose and xylose liberated from cellulose and hemicellulose in corn stover were rapidly fermented to ethanol by S.

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  • 目的探讨重组酵母乙肝疫苗新生儿接种后抗体应答持续时间,强化复种必要性

    Objective:To investigate the antibody response to hepatitis B vaccination and the necessity of reinforcement of recombinant hepatitis B vaccine (RHBV) at 12 month.

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  • 重组酵母经冻干能有效地实现在非水相中全细胞催化乙醇酯化合成己酸乙酯。

    We constructed a CALB-displaying yeast whole-cell biocatalyst and applied it to esterification from caproic acid and ethanol.

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  • 目的确定国产乙肝血源疫苗低剂量免疫保护持久性重组酵母乙肝疫苗加强免疫免疫应答。

    Objective To determine the persistence of immune protection of China-made, plasma-derived hepatitis B vaccine in infants and the time table of booster immunization.

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  • 重组培养利用荧光显微镜检测重组酵母荧光强度,然后通过荧光分光光度计检测分选底物相互作用后产生游离的EGFP荧光强度进而确定分选酶的酶活。

    After cultivation, recombinant cells were verified with fluorescence microscopy and sortase activity was detected by fluorescence spectrophotometer from variety of free EGFP's fluorescence intensity.

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  • 重组酵母中进一步表达特异性蛋白

    The recombination EgB can be used for the expression of specific protein in yeast.

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  • 采用ELISA夹心法检测重组IL - 2酵母表达产物免疫学活性

    Sandwich ELISA Method for measurement of the activity of recombinant human IL-2 expressed in yeast was established.

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  • 结果获得了同源重组整合酵母染色体上的能有效利用甲醇作为唯一源的PMD11PMD16转化株。

    Results the PMD11 and PMD16 transformants were obtained, capable of metabolizing methanol as sole carbon resource through non homologous integration into the yeast chromosome.

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  • 本文实现了重组蓝蛋白基因在毕赤酵母中的异源表达

    In this paper, the heterogeneous expression of recombinant allophycocyanin in Pichia pastoris was completed.

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  • 评价接种酵母重组乙肝疫苗(基因疫苗)免疫效果免疫持久性,基因疫苗推广应用及高危新生儿乙型肝炎的预防提供依据。

    To assess the immunogenecity and efficacy of the yeast recombinant hepatitis B vaccine (YHB vaccine) for the prevention of hepatitis B in the newborns.

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  • 因此推荐肝癌细胞代谢活化重组基因酵母检测方法相结合,用于环境样品直接和间接雌激素效应物质的检测。

    Therefore it is recommended to combine the yeast assay with the metabolic activation method based on H4IIE cell line to assay the direct and indirect estrogenic chemicals in environmental samples.

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  • HEV结构区ORF2蛋白甲醇营养型酵母中的成功表达以及初步纯化得到具有强免疫学活性的重组蛋白,研制新型戊型肝炎基因工程疫苗奠定了基础。

    The successful expression of HEV ORF2 protein in p. Pastoris and the production of recombinant protein provides basis for genetically engineered vaccine development of hepatitis E.

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  • 提供了涉及酵母产生重组蛋白方法组合物。

    Methods and compositions are provided relating to production of recombinant protein in yeast.

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  • 纯化重组粉酶其他生物化学性质与海洋季毕赤酵母中的野生型菊粉酶相一致。

    Other biochemical characteristics of the purified recombinant inulinase were the same as those of the native inulinase produced by the marine derived P. guilliermondii strain 1.

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  • 结论毕赤酵母系统重组表达联蛋白V具有较高的结合外露PS红细胞的能力。

    Conclusion The annexin V expressed recombinantly in pichia pastoris show higher affinity to PS exposed erythrocytes.

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  • 目的:利用巴斯德毕赤酵母表达系统获得重组层黏连蛋白LG1-3组件蛋白,进一步研究LG1-3的结构和功能间的关系奠定基础。

    Objective: To express the LG1-3 module of human laminin alpha 4 chain in P. pastoris expression system for studying the function of LG1-3 module.

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  • 为了获得大量有活性重组蛋白酶便于今后工业应用,我们将蛋白酶WF146克隆到分泌性表达宿主枯草芽胞杆菌巴斯德毕赤酵母

    For the convenience of production and purification, Bacillus subtilis and Pichia pastor is were used as hosts for the expression of protease WF146.

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  • 为了获得大量有活性重组蛋白酶便于今后工业应用,我们将蛋白酶WF146克隆到分泌性表达宿主枯草芽胞杆菌巴斯德毕赤酵母

    For the convenience of production and purification, Bacillus subtilis and Pichia pastor is were used as hosts for the expression of protease WF146.

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