目的是要构建和鉴定耻垢分枝杆菌的重组体。
The objective is to construct and identify recombinant of Mycobacterium smegmatis.
通过酶切、PCR及测序鉴定各重组体。
The recombinants were analyzed and identified by restriction enzyme, PCR and sequencing.
此重组体可在酵母中进一步表达特异性蛋白。
The recombination EgB can be used for the expression of specific protein in yeast.
目的：从DNA重组体中快速鉴定出阳性克隆。
Objective: To identify positive clones from DNA recombinants rapidly.
背景：重组体人红细胞生成素通常用于治疗贫血。
BACKGROUND: Recombinant human erythropoietin is commonly used for treatment of anaemia.
采用电转染方法将重组体导入HL-60细胞中；
The recombinant plasmid was transfected into the cell line HL-60 by electroporation.
委员会建议用同样的观点来考虑在普林斯顿的重组体。
The committee recommended that recombination DNA work at Princeton be considered in this same light.
本发明还涉及包含所述非病毒载体和所述表达型重组体的药物组合物及其应用。
The invention also relates to the pharmaceutical compositions containing the non-viral vectors and the expression type recombinant and their use.
阳离子脂质体法转化COS - 7细胞并经免疫细胞化学染色检测重组体的表达。
It was then transformed into the COS-7 cells by Lipofectin Reagent and its expression was detected by immunocytochemical staining.
由于遗传资源狭窄，有利重组体的数量受到限制，使育种工作进展缓慢，甚至停滞不前。
Because the heredity resources is narrow, the restrict that the quantity of the beneficial reorganization suffer, make the breeding work progress slowly, even bog down.
多巴染色及电镜观察结果表明转染了反义重组体的B16细胞其黑色素颗粒的含量明显低于对照组。
The results of Dopa staining and electronic microscopy showed that the production of melanin also decreased significantly compared with control B16 cells.
该重组体在大豆根上能结瘤，并且从根瘤中分离出的后代品系对盐、抗生素和毒素仍保持同样高的抗性。
The strains isolated from nodules of the recombinant grown on soybean root main-tain the same high tolerance to salt, antibiotics and toxin.
Luger和他的同事随即包装了从Shokat小组得来的修饰过的组蛋白，形成核小体和染色质的重组体。
Luger and her colleagues next packaged the modified histones they received from Shokat's group into recombinant nucleosomes and chromatin.
血清学和基因分析显示韩国H1N2病毒亚型直接自美国传入并且韩国没有出现H1N1和H3N2之间的重组体。
Serologic and genetic analysis indicated that the Korean H1N2 viral subtypes were introduced directly from the United States, and did not arise from recombination between Korean H1N1 and H3N2.
数据显示H3N2重组体出现了和欧洲流行的猪流感病毒H3N2的类人HAH3基因以及欧洲流行的猪流感病毒H1N2的NAN2基因。
The data demonstrate the emergence of a H3N2 reassortant with the human-like HAH3 gene of prevalent European porcine H3N2 influenza viruses and a NAN2 gene of the European porcine H1N2 viruses.
我们以这些片段开始，然后通过同源重组将其组合到染色体中。
We start with these fragments, and then we have a homologous recombination system that reassembles those into a chromosome.
纬卡代码正是辐射体代码；此外为了清除和重组该层面的畸变，它们控制着辐射体胶囊的开启和关闭。
Veca codes are Radial Body codes; in addition to clearing and realigning distortions at this level, they control the opening and closing of the Radial Body capsules.
欧元区国家势必会为将来的债务重组付出更大的代价，财政一体化此时更具吸引力。
Future debt restructuring will be more costly for European states and fiscal integration more attractive.
而一旦细胞的抗癌防御系统被摧毁，遗传混乱就会随之而来，于是又进一步加剧了基因突变的程度，而且染色体中的DNA将产生大规模重组。
Once the cell’s anticancer defenses are destroyed, genetic mayhem ensues, with further mutations and wholesale rearrangements of DNA in the chromosomes.
Page推测，这个综合症可能是由于Y染色体重组时出错，导致染色体中不小心出现了一段中心没有空隙的回文。
The syndrome may be the result of Y chromosome recombination gone awry, Page speculates, when the chromosome inadvertently becomes a palindrome with no gap in the center.
目的：构建弓形虫棒状体蛋白（ROP2 ）基因重组质粒并在大肠杆菌中表达，用于筛选弓形虫新的诊断抗原和疫苗分子。
Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
细胞工程主要包括体细胞融合，核移植，细胞器摄取和染色体片段的重组等。
The works mainly comprise the body cells, cell fusion, nuclear transplantation, cell uptake and the reorganization of chromosome segment.
对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body.
目的构建弓形虫棒状体蛋白2 （ROP2 ）基因重组质粒。
Objective To construct a recombinant plasmid containing rhoptry protein 2 (ROP2) gene of Toxoplasma gondii .
对大肠杆菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。
Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E.
遗传学的连锁分析是指通过遗传重组来研究染色体的结构，定位基因的位置，并确定基因在染色体上的排列顺序。
Genetic analysis of linkage used recombination to analyze the structure of chromosomes, to determine the locations of genes and their linear order along a chromosome.
这意味着减数分裂中同源染色体不能配对，染色体间形成的交叉也会更少（例如，在减数分裂早期的重组位点）。
This means that homologous chromosomes failed to pair together during meiosis and chromosomes formed fewer crossovers, i. e. sites where they recombine, during the initial stages of meiosis.
测定了猪生长激素基因工程菌不同表达效率时细菌总蛋白的含量以及包涵体中重组猪生长激素占包涵体总蛋白的比值。
We determined the content of the total bacterial proteins and the proportion of recombinant porcine growth hormone(rpGH)in inclusion bodies(IBs)in the cultures of genetically engineered rpGH E.
重组蛋白技术在许多领域已成为一种必要的工具，但有时表达的目的蛋白却未进行正确的折叠而被蛋白酶降解或聚集形成包涵体。
Many recombinant proteins fail to fold into native state with activity when expressed in Escherichia coli, instead, they often misfold and are easy to accumulate as inclusion bodies.
重组蛋白技术在许多领域已成为一种必要的工具，但有时表达的目的蛋白却未进行正确的折叠而被蛋白酶降解或聚集形成包涵体。
Many recombinant proteins fail to fold into native state with activity when expressed in Escherichia coli, instead, they often misfold and are easy to accumulate as inclusion bodies.
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