方法:将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中,研究表达水平的差异。
Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.
表明可合并组成一段含启动子、起始密码子和NKND四个氨基酸的678个碱基的单克隆抗体M26~32抗原决定簇基因片段。
A combined 678 bp sequence which containing promoter, initiation codon and NKND sequence can be used for further research of target antigen gene of pan species monoclonal antibody M26~32.
标明转译起始位置的密码子。
翻译起始区(TIR)的二级结构是影响翻译效率的决定性因素,同时密码子的偏好性问题也是个至关重要的方面。
The secondary structure of the translation initiation region (TIR) is a determinant factor for translation initiation rate, meanwhile, codon preference plays an important role, too.
翻译起始区(TIR)的二级结构是影响翻译效率的决定性因素,同时密码子的偏好性问题也是个至关重要的方面。
The secondary structure of the translation initiation region (TIR) is a determinant factor for translation initiation rate, meanwhile, codon preference plays an important role, too.
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