结果突变基因重组表达质粒构建正确；
Results The recombinant plasmid with mutant gene was constructed correctly.
结论成功构建了重组人FHIT真核表达质粒。
Conlusion The human FHIT recombinant eukaryotic expression plasmid was constructed successfully.
然后经真核表达质粒-脂质体介导，导入C2C 12细胞系。
After mediated by eukaryotic expression plasmid-liposome, the fused protein was introduced into C2C12 cell system.
目的：克隆大鼠神经营养因子4全长基因，构建真核细胞表达质粒。
AIM: to clone rat neurotrophin-4 (NT-4) total gene and construct expression plasmid for prokaryotic cells.
序言真核表达质粒的构建和转染是目前医学研究中常用的分子生物学手段。
Introduction The construction and transfection of eukaryotic expression plasmid are commonly molecular biology method used in medical research.
目的观察间质胶原酶(MMP - 1)表达质粒对大鼠肝纤维化的影响。
Objective To observe tile effects of MMP-1 (Matrix Metalloproteinase-1) expressing plasmid on rat liver fibrosis.
目的：构建食管癌相关基因2ECRG2原核表达质粒，在大肠杆菌e。
Objective: to construct a prokaryotic expression vector containing esophageal cancer related gene 2 ECRG2 and observe its expression in Escherichia coli e.
将正确连接的表达质粒线性化后用电穿孔法转化到毕赤酵母GS115中。
The correct plasmid was linearized and transformed into Pichia Pastoris strain GS115 by electroporation.
目的构建人胰岛素样生长因子1 (IGF - 1)的真核细胞表达质粒。
Objective to construct eukaryotic expression plasmid for human insulin-like growth factor 1 (IGF-1).
相应地将构建的表达质粒导入变铅青链霉菌TK24中获得五株基因工程菌株。
These plasmids were introduced into S. lividans TK24, respectively and five genetic engineering strains were constructed.
结论：表达质粒的拷贝数、宿主菌培养条件、细胞破碎方式等均能影响酶的表达量。
Conclusion: The culture conditions and procedure of cell disrepution can influence the expression of GDH.
结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。
Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
该腺病毒和真核表达质粒在肿瘤治疗药物、放射治疗增敏的制备中具有独特的实用价值。
The adenoviruses and the eukaryotic expression plasmid have unique practicality in preparing tumor treating medicines and radiotherapy hypersensitivity.
构建小鼠il -4截短型基因真核表达质粒，表达小鼠il - 4受体拮抗体蛋白。
To clone mouse interleukin 4 (mIL-4) truncated gene, construct its eukaryotic expression plasmid pFB-mIL4 and express the truncated protein (murine IL-4 receptor antagonist).
目的：构建CD 147反义rna表达质粒载体，探索治疗骨肉瘤侵袭和转移的新方法。
AIM: to construct eukaryotic antisense RNA expression vector of CD147 and probe into a new method to treat invasion and transfer of osteosarcoma.
将筛选的抗体模拟肽与TSST - 1突变体基因融合或化学耦联，构建重组表达质粒。
Through crosslinking the specific peptide and the gene of TSST-1, we got the recombination plasmid.
首次构建了原核表达质粒，成功地实现其在大肠杆菌中的大量表达和表达产物的免疫原性分析。
Our works is the first time to construct the recombinant plasmid and successfully expressed the recombinant protein in Escherichia coli, then analyzed the antigenicity of the protein.
结论克隆BLCAP基因并重组原核细胞表达质粒，为分析蛋白特性、研究基因功能创造了条件。
Conclusions: it is very helpful to clone and construct the recombined plasmid of BLCAP gene and to study the qualities of protein and the function of BLCAP gene.
结论SEA基因成功克隆到表达质粒内并表达，为制备单抗、诊断试剂及其致病机制研究奠定了基础。
Conclusions the success of cloning and expression of SEA in E. coli provides basis for study the preparation of monoclonal antibody and diagnostic reagent.
为了确定这个相互作用，我们构建了能表达带有GST和His6标签的全长Arx的原核表达质粒。
To confirm this interaction, we expressed full-length of mouse Arx tagged with either His6 or GST in bacteria.
结论：高免疫原性的抑制素表达质粒的构建为利用抑制素基因免疫技术诱导单胎动物生多胎奠定了基础。
Conclusion: the fusion gene expression vector was successfully constructed, and it set up the basis of inhibin gene immunization to induce multiple bear for single birth animals.
方法构建了不同TPR串联簇的截断突变体的表达质粒后，建立了稳定转染的BEL740 2细胞株。
Methods the plasmids which can express the truncated mutants of the TPR tandem clusters at different region of CRLP were constructed, stable transfected BEL7402 cell lines were established.
目的克隆人促甲状腺激素受体胞外段基因，构建重组真核表达质粒，获得具有免疫学活性的纯化重组蛋白。
Objective to clone and construct the plasmid containing human thyroid stimulating hormone receptor (TSHR) gene ectodomain, and then identify the immunoreactivity of the purified recombinant protein.
目的构建癌胚抗原（CEA）与热休克蛋白70（HSP70）的真核双表达质粒，并检测其在体外的表达。
Objective To construct the eukaryotic coexpression plasmid encoding carcinoembryonic antigen(CEA) and heat shock proteins 70 (HSP70), then detect the expression of the plasmid in vitro.
方法：用脂质体转染携带PTTG基因的表达质粒，构建过表达PTTG基因的垂体细胞株，观察细胞形态。
Methods: Transfect plasmid which carries PTTG gene use liposome, constructs the pituitary cell line which high express PTTG gene, observe the cell form.
目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
目的观察增殖细胞核抗原(pcna)基因反义rna表达质粒对人胃癌细胞生长特性的影响，探讨其抗肿瘤作用。
Objective To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell.
结果构建了具有正确基因序列的CFP10重组表达质粒，重组蛋白在大肠杆菌BL21（DE3）中以包涵体形式表达。
The recombinant CFP10 protein was expressed in inclusion body in E. coli BL21(DE3), and the target gene had been cloned into host bacterium.
方法 将绿色荧光蛋白 （GFP）表达质粒导入 膀胱癌细胞株BTT T739，筛选出高表达GFP的细胞克隆并配置成细胞悬液。
Methods BTT-T739 cells, a mouse bladder transitional cell carcinoma cell line, were tranfected with GFP plasmid to screen stable GFP expressing clones.
目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen (WHcAg) and prepare polyclonal antibodies.
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