目的探索利用乙型肝炎病毒(HBV)作为基因治疗载体的可能性及检验其表达反义rna抗hbv的作用。
Objective to explore the possibility of using HBV as a gene delivery vector, and to test the anti-HBV effects by intracellular expression of antisense RNA.
目的构建反义MBD1基因片段真核表达载体，为研究MBD1基因功能提供工具。
Objective to construct eukaryotic expression vector of antisense MBD1 gene fragment and to provide a tool for studying MBD1 gene function.
目的观察vegf反义寡核苷酸对膀胱癌细胞VEGF表达的抑制情况。
Objective To study inhibition of VEGF expression in human bladder carcinoma cells by antisense oligonucleotides.
结果VEGF反义寡核苷酸对膀胱癌细胞的VEGF表达有明显的抑制作用。
Results VEGF antisense oligonucleotides obviously inhibited VEGF expression in human bladder carcinoma cells and proliferation of endothelial cells.
结果：转染后反义rna均得到高水平表达，并使NK细胞的杀伤活性提高约15%。
Results: After the transfection, antisense RNA had been successfully expressed respectively, and NK cells activity had been improved about 15%.
目的：研究细胞间粘附分子- 1 (ICAM - 1)反义dna对内皮细胞ICAM - 1表达的抑制作用。
Objective: to observe inhibitory effect of intercellular adhesion molecule1 (ICAM1) antisense DNA on ICAM1 expression in endothelial cells.
目的：构建CD 147反义rna表达质粒载体，探索治疗骨肉瘤侵袭和转移的新方法。
AIM: to construct eukaryotic antisense RNA expression vector of CD147 and probe into a new method to treat invasion and transfer of osteosarcoma.
目的探讨针对乙型肝炎病毒（HBV）x、p双靶区反义RNA对乙型肝炎病毒转基因小鼠HBV复制和表达的影响。
Objective To investigate the effect of dual-target antisense RNA of hepatitis B virus (HBV) targeted to X and P region on replication and expression of HBV gene in HBV transgenic mice.
有研究表明P 33ing1的过表达可促进由“血清饥饿”诱导的细胞凋亡，而转染反义rna则可抑制“血清饥饿”导致的细胞凋亡。
It was investigated that over-expression of P33ING1 could promote apoptosis induced by lack of serum, and transfection antisense RNA could suppress apoptosis induced by lack of serum.
反义组、诱骗组、反义加诱骗联合干预组增殖细胞核抗原表达显著低于模型组，差异均有显著性意义（P<0.05）。
The expression of PCNA in the antisense group, decoy group and antisense plus decoy group were obviously lower than that in the model group, and the differences were significant(P< 0.05).
所以导入MDR1基因硫代反义寡聚脱氧核苷酸可以部分抑制SKOV3/mdr1细胞中MDR1基因的表达。
So MDR1 expression in SKOV3/mdr1 cell line was partially inhibited after MDR1-AS treatment in the mRNA and protein level.
将反义词放在一起表达一些不会发生的事是非常好的方式：这个用法最早可以追溯到乔叟的《坎特伯雷故事集》。
Putting oppositestogether to cover a spectrum is a pretty good way to indicate that something isn'tgoing to happen: this one can be found as far back as Chaucer's CanterburyTales.
目的了解反义乙型肝炎病毒（HBV）基因转移、表达的抗HBV作用。
Objective To explore the effect of antisense RNA on hepatitis B virus (HBV) replication and antigen synthesis.
目的研究内皮素转换酶-1（ECE -1）反义RNA对人气道上皮细胞ECE -1表达的抑制作用。
Objective To explore the effects of endothelin converting enzyme-1 (ECE-1) antisense RNA on ECE-1 expression in human bronchial epithelial cell line(16HBE).
结果：卵巢癌细胞转染反义ERCC1基因后，ERCC1基因转录水平、蛋白表达水平明显下降。
Results: After transfection of the antisense ERCC1, Northern and Western blotting indicated that the RNA and protein expression level of ERCC1 was obviously decreased.
反义rna广泛存在于各类生物中，它作用于DNA的复制、转录、转译的过程，实现对基因表达的调控。
Antisense RNA exists in all kinds of living beings wildly. It ACTS on the course of the replication of DNA, transcription and translation, which realize the regulation and control of gene expression.
并构建针对集聚蛋白的反义质粒，从而进一步研究下调集聚蛋白的表达对淋巴细胞活化的影响。
Constructed antisense plasmids were used to explore function of agrin in the proliferation of lymphocyte activation.
目的：构建反义vegf基因真核表达载体，研究其对肾癌细胞VEGF表达的影响。
AIM: to construct eukaryotic expression vector carrying human antisense VEGF gene and to study its effect on VEGF expression and growth of renal cell carcinoma.
结论转染反义端粒酶RNA在封闭细胞端粒酶活性表达的同时，伴发P21与P16表达水平的升高。
Conclusion Antisense telomerase RNA can block telomerase activity in SMMC7721 cells accompanied simultaneously with an increased P16 and P21 expression levels.
方法：检测反义阻断BHRF1表达后BHRF 1 -CNE2细胞在喜树碱作用下增殖能力和凋亡指数的变化。
Methods: After incubated with BHRF1 antisense oligonucleotide, BHRF1 CNE2 cells were treated with camptothecin, and tested for the alterations of proliferative ability and apoptotic index.
本研究克隆了苹果NAD-SDH 基因的部分cDNA 序列，构建了其反义表达载体，并正在通过农杆菌浸染法将该基因反义转化至蔷薇科植物中。
In this dissertation, a partial fragment of NAD-SDH from apple fruit was cloned, the NAD-SDH antisense expression plasmid was constructed, and the antisense expression has been doing;
目的观察增殖细胞核抗原(pcna)基因反义rna表达质粒对人胃癌细胞生长特性的影响，探讨其抗肿瘤作用。
Objective To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell.
目的观察增殖细胞核抗原(pcna)基因反义rna表达质粒对人胃癌细胞生长特性的影响，探讨其抗肿瘤作用。
Objective To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell.
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