目的对怀地黄茎尖培养苗进行病毒学鉴定。
Objective To identify if the tobacco mosaio virus remains of plantlets from shoot-tip culture of Rehmannia glutinosa.
为了克服这一缺点，现在经常是将茎尖培养与热处理相结合来使用。
In order to overcome this shortcoming, the tip is now often a combination of training and heat treatment to use.
技术上通常切取茎尖越小，脱毒效果越好，但是茎尖培养成活率变低。
Technical tip is usually the smaller the cut, the effect of virus-free as possible, but low survival rate of shoot tip culture.
不同光质在河套蜜瓜茎尖培养中，以红兰混合光对嫩茎的形成有明显的效果。
Mixing light had great effect on the formation of young stem in meristem culture.
热处理、茎尖培养、化学制剂处理及其联合应用为梨病毒脱除提供了有效途径。
Using thermotherapy, meristem culture and chemical preparation may provide effective ways for obtaining virus-free materials of pear.
茎尖培养中最主要的影响因素就是切取茎尖的大小，一般要求茎尖长度小于1mm。
Shoot tip culture in the most important factor is the impact of the cut tip size, tip length is less than the general requirements of 1mm.
以茎尖培养获得的红梗芋试管苗和试管球茎为材料，在国内首次研究芋试管苗和试管球茎连续两年大田种植的生长、产量和品质情况。
Study on growth, yield and quality of plantlets and mocrocorm in vitro of Colocasia esculenta L. Shott cv. HY planted in continuous two years.
研究了甘蓝的芽尖和茎与腋芽的组织培养技术。
The tissue culture technology about shoot-tip and stem with auxiliary bud of Kalanchoe Blossfeldian were studied.
利用茎尖组织培养便可获得无毒或不带某些病毒的植株，进而生产脱毒种薯。
So it was necessary to use virus-free potato plants to produce virus-free seed potato through stem-tips tissue cultivation.
微小的茎尖切段通常是难以培养的。
Minute fragments of shoot apices are proverbially difficult to culture.
采用茎尖分生组织培养技术，获得分蘖洋葱无毒试管苗。
Virue free in vitro shoots of Tillered onion were obtained from meristem tip apex tissue culture.
将海岛棉茎尖分生组织经过3～6周的诱导、继代培养生长后，可以形成足够量的再生植株。
Sufficient plantlets of regeneration were produced within 3~6 weeks induced and culture from apical meristems.
以蝴蝶兰试管苗茎尖为外植体，接种于添加不同浓度激素配比的1/2 MS培养基上进行原球茎诱导。
The stem tips of phalaenopsis test-tube shoot were used as explants to be cultured in the medium(1/2MS) with different concentrations of hormones.
在茎尖再生体系建立的过程中，不同培养基上外植体丛生芽发生的方式和芽尖倍增数均不同。
In the process of established regenerate system from shoot meristems, the mode of tussockybud occurring and multiplication of shoot meristems were all different.
芦笋的花药、花粉、茎尖及原生质体经离体培养均可再生植株。
The anther, pollen, stem point and bioplast cultured in vitro can be plant.
采用茎尖分生组织培养技术，获得了川麦冬的脱病毒试管苗。
Virus-free materials of Ophiopogon japonicus were obtained from meristem culture in vitro.
毛樱桃茎尖在MS，G，L三种培养基上均可增殖生长，但以在L培养基中生长较好。
Manchu cherry shoot tips can propagate on such three kinds of mediums as MS, G and L mediums, growing vigorously on L medium.
由茎尖分生组织培养成无病毒苗并进行试管保存，是当前既经济又实用的方法。
Meristem tip culture of Sweetpotato can obtain virus-free plantlet. Storing the virus-free plantlet in vitro is economic and applicable at present.
以豫薯4号和豫薯12号为试材，研究了生长素类物质（IBA和NAA）、温度和光照时间对转移后甘薯茎尖分生组织培养成苗的影响。
Yushu 4 and Yushu 12 were used to study the effects of auxins(IBA and NAA), temperature and illumination time on plantlets regeneration from subcultured tip meristem in sweet potato.
以东方百合品鳞片为外植体，采用MS培养基和不同外源激素配比，进行组织培养，研究其茎尖分生组织分化。
Meristem culture of Oriental Hybrid Lily through its scales was studied in MS medium with different external hormone treatments.
采用茎尖分生组织培养脱毒，繁殖、使用脱毒种薯是目前世界上解决种薯退化、提高单产的有效和主要途径。
The major and efficient way to promote potato yield and control the degradation of potato seed tubers in the world is to use the virus-free seed tubers propagated via shoot meristem culture.
以带芽茎段、茎尖做外植体，建立了红叶乌桕的组织培养和再生体系。
The explants from Euphorbia cotinifolia(L. ) stem fragments and shoot tips were used to establish culture in vitro and regeneration system.
以柠檬天竺葵的叶片、茎尖为外植体进行试管培养，结果表明，茎尖诱导分化的效果较好。
The leaves and stem segments of lemon-scented geranium were cultured in tubes as explants.
以红实美草莓匍匐茎剥取茎尖，不同大小茎尖接种到不同配方的茎尖诱导培养基中。
Taking the stem points of the red solid beautiful strawberry, the different big and small stem points are vaccinated in the different formula stem point induction culture medium.
香石竹红色品种茎尖试管苗继代培养玻璃化现象是香石竹脱毒试管苗生产的一个主要障碍。
Vitrification is the main obstacle in subculture of carnation plantlets in vitro, which were induced from shoot tips.
在改良MS培养基中加入几种中草药剂处理，研究对葡萄茎尖组培苗生长势的影响。
The extracts of several Chinese medicinal herbs were separately added into the improved MS culture medium to study the effects on the growth of grape stem- apex in vitro.
以长寿花为材料，研究了其茎尖和带腋芽茎段组织培养技术。
The tissue culture technology about shoot-tip and stem with auxiliary bud of Kalanchoe Blossfeldian, which were used as explant, were studied.
以大白杜鹃茎尖及茎段为试验材料，研究了基本培养基及激素配比等对大白杜鹃芽的诱导增殖的效果。
The stem tips and segments of Rhododendron decorum Franch were cultured on 4 different basic media plus different proportion of hormones to investigate its abduction and multiplication effects.
以大白杜鹃茎尖及茎段为试验材料，研究了基本培养基及激素配比等对大白杜鹃芽的诱导增殖的效果。
The stem tips and segments of Rhododendron decorum Franch were cultured on 4 different basic media plus different proportion of hormones to investigate its abduction and multiplication effects.
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