花粉管经由一个助细胞进入胚囊；
The pollen tube entered into the embryo sac via one synergid.
对大豆花粉管通道技术进行深入研究。
花粉管在柱头内逆行生长；
血清反应阻止花粉管生长。
认为由花粉管的流出物能使珠心细胞活化。
Activation of nucellar cells by discharge from the pollen tubes has been claimed.
花粉管细小或畸形；
实验结果说明花粉管的定向生长受胚珠的分子信号调控。
It is suggested that directional growth of pollen tubes are controlled by molecular signal in ovule.
利用花粉管通道技术对上述基因进行了黄瓜的遗传转化实验。
PPN-At2 formed. 3 Cucumber transformation test was carried for bellow genes by Pollen-tube pathway.
较高浓度处理可使花粉管形态趋向异常，细胞质流动趋于停滞。
Moreover, higher concentrations also tended to disturb tube morphology and cytoplasmic streaming.
当生殖细胞进人花粉管后，在较短的时间内接近营养核，并与其相贴合。
After entering the pollen tube, the generative cell approached the vegetative nucleus before long and sticked each other.
自1983年我国学者创立花粉管通道法以来，在多种农作物中得到应用。
The method of pollen tube pathway was formally established in 1983. Since then, it has been widely used in crop genetic improvement.
以黑松花粉粒和花粉管为试验材料，研究了离子注入对细胞骨架系统的损伤效应。
The damage of the cytoskeleton system in the pollen and the pollen tube of Pinus thunbergii induced by ion beam implantation were researched.
花粉管的生长过程包括许多方面，其中最为重要的是花粉管细胞壁的合成和胞质运动。
The process of pollen tube growth involves many aspects, especially the synthesis of cell wall and the cytoplasmic movements.
即使父本花粉管进入母本柱头，因生长缓慢，沉积胼胝质，在花柱中受抑制而停止生长。
Secondly, if pollen tubes entered into stigma, growth was retarded with callose plug deposition, then they stopped to grow because of inhibition.
主要研究鸢尾在不同贮藏条件下保存的花粉活力的变化，以及温度对花粉管萌发和生长的影响。
The variation of pollen vigor of Iris in the different storage conditions was studied and the germination and the growth of pollen tube in different temperatures were also studied.
文章就植物花粉管、保卫细胞中微丝骨架以及微丝骨架与信号转导的一些研究进展作了简要介绍。
The advance on microfilament cytoskeleton in plant pollen tube and guard cells, and signal transduction involving in plant microfilament cytoskeleton were simply illustrated in this paper.
一定浓度的蔗糖溶液有助于花粉管的生长，10%、20%蔗糖浓度均能显著提高花粉管伸长速度。
Certain concentration of sucrose solution is helpful to growth of pollen tube; both 10% and 20% sucrose solutions can significantly increase elongation velocity of pollen tube.
实际转化操作表明，子房滴注转化法的PCR阳性率（4.5%）要高于花粉管通道法（2.0%）。
More PCR positive plants could obtain by ovary-drip (4.5%) than that by pollen tube pathway (2.0%), when transformation was conducted.
本文利用花粉管通道在大豆自花受粉后，将野生大豆DNA导入栽培大豆，从而引起了后代性状的广泛变异。
This study reports that wild soybean DNA was introduced into cultivar soybean through pollen tube after soybean self-fertilizing, it led to wide variation in offspring characteristics.
阐述了化学导入法(PEG)、电穿孔法、注射法，粒子轰击法、花粉管萌导法及使用其它载体系统的成功实例。
The direct gene transfer systems involved the chemical method (PEG), electroporation, microinjection, particle bombardment, pollen-mediated gene transfer and other systems using various vectors.
采用花粉管通道法遗传转化技术，将慈姑蛋白酶抑制剂基因API导入水稻品系“9311”，获得了转基因植株。
Transgenic rice plant was obtained by transferring the Arrowhead Proteinase Inhibitor (API) gene into rice line "9311" by pollen-tube pathway method.
在液滴培养盐胁迫条件下，棉花花粉粒萌发的百分率是反映其耐盐性的可靠指标，而花粉管的长度不能反映棉花的耐盐性。
The pollen germination percentages of the same 8 cultivars by sitting drop culture were also proved to be reliable indexes of salt tolerance, but pollen-tube growth were not.
本文采用聚丙烯酰胺凝胶电泳的方法，对利用花粉管通道途径，导入外源DNA所获大豆变异后代，进行过氧化物酶酶谱分析。
With the method of polyacrylamide gel electrophoresis analysis of the peroxidase patterns in variant progenies of soybean obtained by introduction of DNA through pollen tube.
这些结果证明供体燕麦上可能携带抗小麦条锈菌的新基因，并已通过花粉管通道进入小麦，整合到小麦的染色体组中，稳定表达。
The result also indicated that the new genes resistant to yellow rust may be carried on oat DNA, which have been integrated into the genome of wheat and expressed steadily.
结果表明 ：几丁质酶对镰刀菌引起的西瓜枯萎病有一定的抑制作用 ，利用花粉管通道法直接导入西瓜活体植株的技术是可行的。
The results indicated that the chitinase had certain inhibition effect on Fusarium oxysporum, and the method of pollen tube pathway could be successfuly used in watermelon transformation.
CTX处理柱头后自花授粉对自花花粉管的生长具有促进作用；而PTX处理柱头后异花授粉，表现出对异花花粉管生长的抑制作用。
Self-pollen tube growth could be promoted by treating stigma with CTX, but the pollen tube growth was inhibited by treating stigma with PTX before cross pollination.
CTX处理柱头后自花授粉对自花花粉管的生长具有促进作用；而PTX处理柱头后异花授粉，表现出对异花花粉管生长的抑制作用。
Self-pollen tube growth could be promoted by treating stigma with CTX, but the pollen tube growth was inhibited by treating stigma with PTX before cross pollination.
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