方法采用膜片钳全细胞式记录技术。
METHODS The patch clamp whole cell recording technique was used.
方法采用细胞贴附式膜片钳技术。
方法应用膜片钳全细胞记录模式。
方法：膜片钳全细胞电流记录方法。
Methods: Patch clamp whole cell recording technique was used.
方法：采用全细胞膜片钳方法。
方法：采用制霉菌素穿孔膜片钳技术。
Methods:Using nystatin perforated patch clamp recording configuration.
方法采用全细胞膜片钳技术。
方法采用全细胞膜片钳技术。
方法：采用细胞贴附式的膜片钳技术。
METHODS: The date recording from cell - attached patches of patch - clamp technique.
方法膜片钳全细胞记录。
方法全细胞膜片钳技术。
方法采用全细胞膜片钳技术，进行电流钳制。
Methods The whole-cell patch clamp with current clamp was used.
膜片钳技术已成为细胞电生理学研究的重要手段。
The patch clamp technique has become the main method for cell electrophysiology research.
膜片钳技术已成为分子和细胞学研究的重要手段。
The patch clamp technique has become the main method for the molecule and cell research.
方法：采用全细胞膜片钳技术，记录钠通道电流。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
膜片钳技术给动脉粥样硬化发病机理研究带来了新的亮点。
The patch clamp technique bring about new light set in studying on mechanism of atherosclerotic development.
采用全细胞膜片钳技术，记录动作电位和上述各离子流。
We used current-clamp technique to record action potentials, and voltage-clamp technique to record ionic currents.
膜片钳技术的广泛应用极大推动了人们对离子通道的认识。
The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.
结论：膜片钳技术使探讨烟碱受体拮抗药的作用位点成为可能。
CONCLUSION: the patch clamp technique makes it possible to observe the action sites of nicotine receptor antagonists.
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
应用此系统我们建立了稳定的人心肌细胞离子通道膜片钳记录方法。
An stable method for human myocytes ion-channel patch-clamp recording were established.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
方法在离体培养的A549细胞上，用膜片钳全细胞模式记录氯通道电流。
Methods the chloride channel currents were recorded in cultured A549 cells by using the whole-cell mode of patch clamp techniques.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法：通过膜片钳手段，测量未分化的SHSY5Y细胞钙离子通道电流；
Methods: By using patch clamp technique, measure voltage gated calcium currents in undifferentiated SH SY5Y cell line.
膜片钳技术是一种先进的电生理技术，在生命科学研究中已得到了广泛的应用。
The patch clamp recording techniques is an advanced electrophysiological technique, which has been widely used for research in the life science research field.
根据检测目的的不同，微电极主要应用可以分为离子选择微电极检测技术和膜片钳技术。
Because the differences of its application, the microelectrode can be divided into ion-selective microelectrode and patch clamp detecting technique.
方法：运用全细胞膜片钳技术记录持续性钠电流，并观察其在缺氧-复氧模型下的变化。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
结论成功建立了一种简单快速的适用于膜片钳技术的大鼠皮层神经元急性分离改良方法。
Conclusion a simple and fast method for acutely isolating rats cortex neurons suitable for patch clamp is successfully found.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
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