目的：分析胚胎生殖细胞体外原代培养条件，为胚胎生殖细胞建系奠定基础。
OBJECTIVE: to analyze the qualifications for in vitro culture of embryonic germ cell for the purpose to set the foundation of establishing embryonic germ cell system in vitro.
结论：众多的因素可以影响胚胎生殖细胞的培养及生长，建立良好的胚胎生殖细胞体外培养条件十分重要。
CONCLUSION: Numerous factors affect the culture and growth of the embryonic germ cell. So it is extremely important for us to establish a good in vitro culture condition of embryonic germ cell.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
应用推荐