人胚胎成纤维细胞饲养层在ES细胞分离过程中具有较好的作用。
Human embryonic fibroblast feeder played a positive role during the ES cells isolation procedure.
大鼠胚胎成纤维细胞(REF)可以作为培养大鼠胚胎干细胞的滋养层。
Rat embryonic fibroblasts (REF) can be used as feeder cells in cultivating rat embryonic stem cells.
目的探讨建立有效的小鼠胚胎成纤维细胞饲养层，用于胚胎干细胞的培养。
Objective To study the isolation and culture and application of Kunming mouse embryonic fibroblasts(MEF).
结论：滤网过滤改良法可以在较短时间内培养出优质高采的鼠胚胎成纤维细胞。
CONCLUSION: Reformed filter method is proved to be the better method to isolate and culture MEF in short times.
目的：建立ICR小鼠胚胎成纤维细胞饲养层培养体系，用于胚胎干细胞建系及研究。
Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast (MEF) cells for isolation and culturing of embryonic stem (es) cells.
将上述定位的鸡的20个功能基因的BAC克隆分别与利用胚胎成纤维细胞制备的鸭和鸵鸟的中期染色体杂交。
The above BAC clones of 20 chicken genes were hybridized by FISH with duck and ostrich metaphase chromosomes prepared from embryo fibroblasts.
Lee的小组使用一种反转录病毒将荧光基因植入狗胚胎成纤维细胞中，但是他们并不能控制这种反转录病毒植入基因的位置。
Lee's team used a retrovirus to transfer the fluorescent gene to dog fibroblast cells, but they could not control where the virus inserted the gene.
目的在本实验室建立小鼠胚胎成纤维细胞培养方法，并在此基础上用培养的细胞检测氯化镉、甲醛等外来化合物的细胞毒性反应。
Objectives: To set up the methods of mouse embryo fibroblasts culture in vitro and to evaluate the toxicity of xenobiotics such as chloride cadmium and formaldehyde by using these methods.
目的： 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞，优化培养条件，建立饲养细胞系，为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
方法取大鼠胚胎分离成纤维细胞，分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
成纤维细胞是胚胎中胚层起源的间叶细胞。
Fibroblasts are mesenchymal cells which are derived from the embryonic mesoderm.
结果表明：胎鼠成纤维细胞MEF培养组的囊胚率和胚胎细胞数38。
The result showed that the mouse embryonic fibroblasts MEF group achieved significantly higher blastocyst formation rate 38.
结果分别获得了传16代的胎鼠成纤维细胞和传7代的人胎儿成纤维细胞，建立了人胚胎干细胞饲养层的培养体系。
Results Acquired 16 passages in mouse and 7 passages in human and established the culture system of human embryonic stem-like cells.
结果分别获得了传16代的胎鼠成纤维细胞和传7代的人胎儿成纤维细胞，建立了人胚胎干细胞饲养层的培养体系。
Results Acquired 16 passages in mouse and 7 passages in human and established the culture system of human embryonic stem-like cells.
应用推荐