细胞克隆均至少能培养4代以上；
These cell colonies could be cultured at least up to 4 passages.
沈现从事于用动物卵细胞克隆人类干细胞。
She focuses on the cloning human embryonic stem cells using animal eggs.
将培养出肿瘤细胞克隆球给予传代扩增；
天后，通过剖腹产获得体细胞克隆小鼠。
At 19.5 days, the somatic cloned mice were delivered by Caesarean secession.
单细胞克隆培养结果。
目的建立抗原特异性T淋巴细胞克隆的方法。
Objective To establish the method of alloantigen specific t lymphocyte clones.
本发明公开了一种生产体细胞克隆猪的方法。
The present invention discloses production process of somatic cell cloned pig.
经g 418条件培养筛选，获得阳性细胞克隆。
Positive clones were obtained by the selection inG418 conditioned culture.
这些癌变细胞克隆生长成肿瘤，并最终具有转移潜能。
These cancerous cells then grow clonally into tumors and eventually have the potential to metastasize.
骨髓增生异常综合征是一种造血干细胞克隆异常性疾病。
Myelodysplastic syndrome (MDS) is a clonal disorder of the hematopoietic stem cell.
红花细胞克隆的条件培养的植板率是普通平板培养的3.6倍。
The PE of single cell clones in condition culture was 3.6 times as high as in normal plate culture.
方法：采用微量细胞克隆形成法检测CNE-2Z对药物的敏感性。
Method:Microcolony formation assay was used in testing sensitivity of CNE 2Z to DDP.
在现代生物实验室细胞克隆的往往是在一个研究项目的一个组成部分。
In the modern biological laboratory cell cloning is often a component in a research project.
慢性粒细胞白血病是一种造血干细胞克隆性增殖所致的骨髓增殖性疾病。
Chronic myelocytic leukemia is a clonal proliferation of hematopoietic stem cells caused by myeloproliferative disorders.
利用银杏单细胞克隆系研究银杏单细胞生长稳定性与细胞均一性之间的关系。
The relationship between stability of ginkgo single-cells and cells uniformity its was studied by cells-cloning series of ginkgo.
本研究的目的是比较小鼠多功能干细胞克隆与胚胎干细胞系的心脏分化潜能。
The aim of this study was to characterize the cardiac differentiation potential of a murine iPS cell clone in comparison to a well-established murine es cell line.
体细胞克隆在绵羊、山羊、牛、猪等家畜中获得了成功，但目前的克隆效率非常低。
Somatic cell clone has been applied successfully in sheep, goat, bovine, swine and so on.
目的：分析T细胞-急性淋巴细胞白血病(T -ALL)病人的T细胞克隆性。
AIM: To analyze t cell clonality in patients with t cell acute lymphoblastic leukemia (T-ALL).
但是，体细胞克隆的效率仍不理想，关键机制还不清楚，严重制约了该技术的推广。
However, unfortunately, poor cloning efficiency and unclear understanding of key mechanisms underlying nuclear reprogramming seriously limit wider USES of animal cloning.
结论直肠癌肿瘤组织TIL细胞中存在特异性识别肿瘤相关抗原肽的T淋巴细胞克隆。
Conclusion: The clonal expanded cells in the TIL are supposed to be the peptide-specific anti-tumor T lymphocyte.
结果显示：经g 418筛选后的阳性细胞克隆，为高表达VEGF的鼠成纤维细胞模型。
The results showed that the positive clones screened by G418 were mouse fibroblast cells with high expression level of VEGF.
结果成功引入点突变并构建置换型打靶载体，转染es细胞后经药物筛选得到抗性细胞克隆。
The drug resistant cell clones were picked after drug selection. Results The construction of targeting vectors was successful and several drug resistant es cell clones were gained.
目的探索建立受体针对供体抗原特异性T淋巴细胞克隆，转入自杀基因诱导移植耐受的可行性。
Objective to explore the feasibility that the recipient against donor antigen specific t lymphocytes clones are formed, and the suicide genes are induced into the clone.
应用石蜡切片及HE染色技术对体细胞克隆黄牛的肺脏及其细胞类型和形态进行了详细的观察。
The lung of somatic nuclear transfer cattle was observed by optical microscopy and he staining techniques in this paper.
通过显微镜观察和绿色荧光染料CFSE 标记后流式细胞术检测方法来判断T细胞克隆增殖情况；
Proliferation of T cells was observed under inverted microscope and detected after CFSE staining by flow cytometry.
因此本文主要综述了影响体细胞克隆猪的几个因素，涉及胞质受体、供核细胞、显微操作、激活以及重组胚移植。
The paper mainly reviewed several factors on influencing somatic cloning of pigs, which involve in cytoplast recipients, donor cells, micromanipulation, activation and reconstituted embryo transfer.
一年前，黄博士在《科学》杂志上发表论文，宣称他已使用胚胎干细胞克隆出了人类胚胎，这在当时激起了一片兴奋。
Dr Hwang provoked a flurry of excitement a year ago when he published a paper in Science that claimed to have cloned human embryos from embryonic stem cells.
在一定剂量范围内，人外周血淋巴细胞克隆效率与照射剂量呈负相关，HPRT基因突变频率与照射剂量呈正相关。
In the radiation dose range used, the colony efficiency of the lymphocytes was negatively correlated to the radiation dose, while the HPRT mutation frequency is positively correlated.
结论：星形胶质细胞瘤的不同部位有DNA含量不同的细胞克隆存在，证实了星形胶质细胞瘤的DNA含量的确存在异质性。
Conclusion: Cell clons with different DNA content are found in different regions of an astrocytoma, it confirms that DNA content of astrocytoma is heterogenous indeed.
结论：星形胶质细胞瘤的不同部位有DNA含量不同的细胞克隆存在，证实了星形胶质细胞瘤的DNA含量的确存在异质性。
Conclusion: Cell clons with different DNA content are found in different regions of an astrocytoma, it confirms that DNA content of astrocytoma is heterogenous indeed.
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