本研究旨在构建具有猪 MSTN 前肽基因定点突变的真核表达载体。
This research intended to construct a eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene.
目的构建反义MBD1基因片段真核表达载体，为研究MBD1基因功能提供工具。
Objective to construct eukaryotic expression vector of antisense MBD1 gene fragment and to provide a tool for studying MBD1 gene function.
该病毒作为载体，应用在杆状病毒-昆虫真核生物表达系统。
It is applied as a carrier in expression system of granulosis virus insect eucaryotic biology.
目的建立丙型肝炎病毒(HCV)复合多表位基因的真核表达载体，并在COS7细胞中瞬时表达。
Objective to construct an eukaryotic expression vector of compound multi-epitope gene of HCV and express the gene in COS7 cells.
结论野生型和突变型PRPF31基因真核表达载体的构建，为研究PRPF31基因突变引起视网膜色素变性的机制奠定了基础。
Conclusion Construction of the eukaryotic expression vectors of wild type and mutant PRPF31 genes is basic work for research on the mechanisms of retinitis pigmentosa caused by PRPF31 mutation.
目的构建水痘-带状疱疹病毒(VZV)糖蛋白e的真核表达载体。
Objective to construct the eukaryotic expression vector of varicella-zoster virus (VZV) glycoprotein e.
目的：构建人死亡受体5 (DR5)真核表达载体，转染NS - 1细胞，建立稳定转染的NS - 1细胞系。
Objective: to construct eukaryotic expression vector of human death receptor (DR5) and transfect NS-1 cells to establish stable NS-1 cell line.
目的构建人胰十二指肠同源盒(PD X - 1)基因的真核表达载体，观察其在NIH3T3细胞中的表达。
Objective to construct eukaryotic expression vector of human pancreatic duodenal homeobox 1 (PDX-1) gene, and to detect its expression in NIH3T3 cell lines.
实验结果表明：该系统使用合适的真核表达载体提供脊灰病毒结构蛋白的技术路线是可行的。
The result indicated: in this system, the technique of eukaryotic expression vector providing poliovirus capsid protein was possible.
目的构建大鼠il - 10真核表达载体并在大鼠软骨细胞中进行表达。
Results rat IL-10 eukaryotic expression vector had been constructed successfully, and had been transfected into rat chondrocyte.
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
目的建立NK细胞受体nkg2d真核表达载体，通过转染NK细胞系yt，初步探讨NKG2D分子对YT细胞系杀伤功能的增强作用。
Objective: to establish NK cell receptor NKG2D eukaryotic expression vector and investigate on the cytotoxicity of NK cell line-YT transfected with NKG2D.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
目的：构建高免疫原性的抑制素真核表达载体。
Objective: to construct inhibin expression vector with high immunogenicity.
目的构建人黑色素浓集激素1型受体（MCHR1）真核表达载体，转染CHO细胞，建立稳定转染的CHO细胞系。
Objective To construct eukaryotic expressing vector of human melanin-concentrating hormone receptor 1(MCHR1), then to transfect CHO cells with the vector for establishment of stable CHO cell line.
MCHR2；真核表达载体；转染；基因表达。
MCHR2; eukaryotic expression vector; transfection; gene expression.
目的：构建人肝细胞生长因子（HGF）的真核表达载体，并在COS7细胞中进表达。
To construct PCI-hepatocyte growth factor (HGF) expression vector and to detect its transient expression in transfected COS7 cell line.
目的克隆激活转录因子(atf)5，构建其真核表达载体，观察其在细胞中的表达定位。
Objective to clone the gene activating transcription factor 5 (ATF5), construct the expression plasmid and detect the localization of ATF5 in cultivated cells.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
构建GGT1重组真核表达载体，观察GGT1在COS7细胞中的定位。
To construct the eukaryotic expression vector of GGT1 and detect its localization in COS7 cells.
目的：构建反义vegf基因真核表达载体，研究其对肾癌细胞VEGF表达的影响。
AIM: to construct eukaryotic expression vector carrying human antisense VEGF gene and to study its effect on VEGF expression and growth of renal cell carcinoma.
将构建好的兔防御素真核表达载体电击转化椭圆小球藻硝酸还原酶功能缺失突变体。
The eucaryotic NP-1 expression vector was transformed into the NR deficient mutant of c.
HBV靶向核糖核酸酶真核表达载体的构建及其在2.2.15细胞内的表达。
Construction of HBV targeted ribonuclease and its expression in 2.2.15 cell line.
目的构建人G250真核表达载体，建立稳定表达人G250的小鼠黑色素瘤细胞系。
Objective To construct the human G250 eukaryotic expression vector and establish the stable B16 cell line expressing human G250 in mice.
该载 体是一种环状载体，包含原核复制起点、真核复制起点、筛选标记基因和绿色荧光蛋 白基因表达盒；
The vector is in ring shape , comprising a procaryon replication origin, two eucaryon replication origins and selective marker genes, and two green fluorescent protein gene expression cassettes;
该载 体是一种环状载体，包含原核复制起点、真核复制起点、筛选标记基因和绿色荧光蛋 白基因表达盒；
The vector is in ring shape , comprising a procaryon replication origin, two eucaryon replication origins and selective marker genes, and two green fluorescent protein gene expression cassettes;
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