进一步工作是设计特异性引物，将其转化为SCAR标记。
Next work is to design the differential primers and transvert it to SCAR.
并利用特异性引物初步建立了马巴贝斯虫病PCR检测技术。
PCR assay for identification of Babesia equi was developed by specific primers.
根据已报道的马铃薯卷叶病毒基因组序列，设计合成一对特异性引物。
Based on the reported genomic RNA sequence of PLRV, two specific primers were synthesized.
表明此特异性引物可以用于玉米组织内南方锈菌的分子检测及病害的早期诊断。
The specific primers were used to amplify some fungal pathogens of maize and healthy tissues.
目的利用PCR技术，尝试建立特异性引物PCR快速检测肠毒素大肠杆菌的方法。
ObjectiveTo establish the rapid detection method of Enterotoxigenic E. coli(ETEC)by polymerase chain reaction(PCR)technology for characteristic primers.
并把分型结果与采用聚合酶链反应-序列特异性引物（PCR-SSP）获得的结果进行比较。
The results of genotyping were compared with those obtained with the polymerase chain reaction method using sequence specific primers ( PCR-SSP).
方法采用多对型特异性引物-聚合酶链反应检测160例慢性乙型肝炎患者血清HBV基因型；
Methods: Serum samples from 160 cases with chronic HBV infection were collected and tested for HBV genotypes by type-specific primers.
目的：研究建立等位基因特异性引物pcr技术体系，并将其应用于基因单核苷酸多态性研究工作。
Objective: to study and establish an allelic specific primer polymerase chain reaction (ASP-PCR) technique system and to apply this technique to study single nucleotide polymorphism (SNP) of genes.
目的：(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
方法4例因献血感染HCV病人的系列血标本，用特异性引物扩增5'-NCR和核心区基因并进行测序。
Methods Serial sera were collected from 4 plasma-donors with HCV infection. 5' - NCR and core gene were amplified, sequenced and analyzed using software.
结果：本研究合成的45条特异性引物和两条内质控引物，检测70例健康人和43例银屑病患者DNA标本。
Results: DNA samples from 70 healthy people and 43 patients with psoriasis were detected by PCR with 45 specific primers and two primers as internal quality control.
方法收集济南市儿童医院婴幼儿病毒性腹泻粪便标本，使用杯状病毒特异性引物对标本进行RT-PCR检测。
Methods The fecal specimens from children with acute nonbacterial gastroenteritis were collected and HuCV in the samples were detected by reverse transcription (RT)-PCR.
方法提取患者脑脊液和血清中的病毒rna，用肠道病毒特异性引物进行套式rt - PCR扩增，检测特异性基因片段。
Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.
方法：采用序列特异性引物聚合酶链反应的方法对汉族72例uc患者和314例正常对照者HLA - DRB1基因分型。
Methods: By using polymerase chain reaction-sequence specific primers, frequency of HLADRB1 alleles in 72 patients with UC and 314 healthy controls were detected.
结论：本研究成功建立了一种改良hbv型特异性引物pcr基因分型方法，其具有灵敏、准确、快速、经济、国内适用等特点。
Conclusion: The study has successfully established a sensitive, accurate and fast, suitable for domestic application HBV genotype-specific primers PCR typing method.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
利用本实验室自行设计的梨火疫病菌特异性引物REA-FEA，再次验证了免疫吸附-PCR技术的灵敏性和专化性，得到了同样的结果。
Using the specific primers REA-FEA designed in this work by ourselves, we proved the specificity and sensitivity of the immuno-capture-PCR method again. The same result has been achieved.
方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
该研究通过设计合成hla基因序列特异性引物，建立了HLA -DR基因分型的套式扩增和直接扩增pcr -SSP技术，并在骨髓移植配型中进行了应用。
In this study, a nested PCR SSP and a direct amplification PCR SSP protocols for HLA DR genotyping were developed and were used in the selection of matched donor for sibling BMT.
采用顺序特异性引物聚合酶链式反应（PCR-SSP）DNA分型技术，首次对35例肾移植供受者和4份标准DNA进行HLA-DR2、DR7、DR9基因配型。
HLA-DR2, DR7, DR9 genotyping by polymerase chain reaction with sequence-specific primers (PCR-SSP)was carried out for 35 individuals and 4 cell lines DNA.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA，采用RSCA和序列特异性引物体外基因扩增(PCRssp)法平行对照对HLA A基因位点进行分型。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
方法根据HCVH病毒株序列设计、合成序列特异性的引物。
Methods Sequence specific primers were designed and synthesized according to the HCV H strain of virus sequence.
方法以一段特异性通用引物并配合热启动聚合酶链反应技术来检测临床标本中的棘阿米巴原虫。
Methods We used a hot initiated PCR based method with asset of universal acanthamoeba specific primers to detect acanthamoeba.
结论采用通用性强的引物系统配合特异性高的热启动PCR技术检测临床和实验室标本中是否存在病原性真菌的方法有重要的应用潜力。
Conclusion This highly universal primer system in combinaition with highly specific hot initiated PCR might be used in the detection of medically important fungi in experimental or clinical specimens.
设计了特异性较好的引物。
引物是PCR特异性反应的关键。
引物是PCR特异性反应的关键。
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