加入950ul溶菌buffer到细胞中如步骤一再裂解一次。
Add 950 ul lysis to the cell pellet and lyse again as step 1.
并对木霉的竞争、重寄生、抗生、溶菌等拮抗机制进行了初步探讨和研究。
The mechanisms of antagonism of Trichoderma sp. including competition, mycoparasitism, antibody-secretion and cell lysis were investigated primarily.
目的:通过噬菌体特性观察,为了解噬菌体吸附特异性及溶菌机理的分子生物学机制奠定物质基础。
AIM: To understand the specific adsorption and bacteriolysis mechanism by observing the biological characteristics of the bacteriophage.
根据它们相互的亲和关系,可把10个细条病菌菌株划分为6个组,把20个噬菌体划分为7个溶菌型;
Accor ding to their interactions, 10 bacterial strains were classified into 6 susceptible groups and 20 phage strains were classified into 7 different lysogenic types .
目的了解不同人群的霍乱杀弧菌抗体水平和对霍乱的免疫状态,并探讨补体溶菌试验检测技术的应用价值。
Objective To study the cholera vibriocidal antibody levels in different groups of people and the immunity state against cholera, and evaluate the complement-mediated bacteriolysis technique.
利用纤维素酶分解菌的固态发酵法降解高变性脱脂豆粕中的纤维素,研究其对提高豆粕中蛋白质水溶出率的作用。
The solid state fermentation with active cellulase producing strains in increasing the rate of protein dissolved of the high denatured defatted soybean cake was discussed in this thesis.
了解披衣菌如何生长及避开溶小体,将可能提供预防或阻挡感染的新方法。
Understanding how the bacteria grow and avoid lysosomes might suggest new ways to forestall or halt the infection.
目的观察海洋假单胞菌碱性蛋白酶(MPAP)的溶栓和抑制血栓形成的作用,研究其对纤溶、凝血和血小板聚集功能的影响。
ObjectiverTo observe the thrombolysis and anti-thrombosis effects of MPAP; To study the actions of MPAP on blood coagulation, platelet aggregation and fibrinolytic activity.
文中通过检测乳酸菌在不同固体和半固体培养基中的菌落数、菌落大小及溶钙圈大小,以筛选出乳酸菌生长的最佳培养基。
The optimal medium for the growth of lactics was chosen by detecting the number, size of colony and the size of calcium dissolving zone when the bacteria grew in different solid and semisolid medium.
磨砂微粒,酵母菌胞溶产物提取物,奥氏海藻提取物。
The frosting particle, the saccharomycetes butcher dissolves the product extraction, Austria seaweed extraction.
溶小体未能将披衣菌碎片呈现在细胞表面,巡逻的T细胞便不晓得哪个细胞窝藏了入侵者。
If the lysosomes cannot provide bits of the bacteria for display on the cell surface, patrolling t cells will not recognize that a cell harbors invaders.
不过,披衣菌却有办法让进入细胞的囊泡避开溶小体,使披衣菌与受感染细胞的其他部份隔离,而能自由增殖。
But chlamydiae somehow compel their entry vacuoles to avoid lysosomes, enabling the bacteria to proliferate freely while separated physically from the rest of the infected cell.
其他的用于酸化的制剂,也就是所谓的干燥或即溶的酸,是由混合了酸面团浓缩物预糊化面粉或者由乳酸菌预发酵的谷物麦芽汁构成的。
Other preparations for acidification the so-called dry or instant acids consist of pregelatinized flour blended with a sour dough concentrate or of cereal mash prefermented by lactic bacteria.
伴随着溶源菌与非溶源菌接合的噬菌体诱导。
Phage induct ion following conjugation of a lysogenic bacterium with a nonlysogenic one.
酚氧化酶原和溶酶菌均是对虾重要的免疫因子。
Both prophenoloxidase and Lysozyme are important immune factors in shrimps.
山葡萄酒浑浊的主要原因有酵母菌自溶、微生物污染、氧化过度、蛋白质和酒石酸盐过多等。
The main causations for the precipitate in amur grape wine are microzyme self-dissolve, microbial contamination, excessive oxidation and over quantity of tarter muriatic acid etc.
结论溶藻弧菌是本次食物中毒的病原菌。
Conclusion Vibrio Alginolyticus was the germ which caused the food - poisoning.
此方法可以用于重组溶葡萄球菌酶中残余宿主菌菌体蛋白含量的测定。
The method is simple, sensitive and accurate, so the trace of bacteria protein in recombinant lysostaphin products can be detected.
方法以溶组织梭菌作为菌种,确立最佳培养条件;
Methods The best medium was designed to culture Clostridium histolyticum.
利用硫酸盐还原菌生物还原硫酸盐的过程中同时可将重金属化学沉淀为难溶金属硫化物而去除。
Biological reduction of sulfate with sulfate-reducing bacteria (SRB) can chemically precipitate heavy metals and remove the insoluble metal sulfide at the same time.
另外,影响生产酸奶发酵剂时的增菌效果,乳酸菌发酵剂的自溶能破坏发酵成分,并且自溶之后释放的细胞质成分,改变了制品的流变学特性和产品固有的感官特性。
Furthermore, the autolysis of the starter can destruct the fermenting agent and the release of the cytoplasmic contents might alter the rheological and organoleptic characteristics of the product.
考察了微球的制备条件,对其体外溶出、体外抑菌、灭菌实验进行了研究。
The condition of preparation process, dissolution property in vitro, antibacterial activity in vitro and the sterilization experiment were studied.
同时,D1菌对于复合溶藻菌剂的抑制影响要大于T5菌的促进作用。
Meanwhile, the inhibit effect of bacteria D1 was greater than the promote effect of bacteria T5.
菌剂复配方式为分别培养后投加,贫营养菌与溶藻菌的复配比为2:1。
The composite ratio of oligotrophic bacteria and algicidal strain is 2:1, after separately training.
目的:利用基因工程技术,构建表达具溶栓活性的纳豆激酶的大肠杆菌工程菌。
AIM: to construct engineered E. coli strains which can express nattokinase with fibrinolysis activity using gene engineering technology.
结果表明:特定氨基酸缺省对嗜淀粉瘤胃杆菌、溶纤维丁酸弧菌和栖瘤胃普雷沃氏菌的生长限制性作用有所差异。
The results showed as follows: there was a certain limitary role of specific amino acid default on the growth of R. amylophilus, B. fibrisolvens and P. ruminicola.
结果表明:特定氨基酸缺省对嗜淀粉瘤胃杆菌、溶纤维丁酸弧菌和栖瘤胃普雷沃氏菌的生长限制性作用有所差异。
The results showed as follows: there was a certain limitary role of specific amino acid default on the growth of R. amylophilus, B. fibrisolvens and P. ruminicola.
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