聚丙烯酰胺凝胶电泳显示单抗偶联柱层析洗脱物仅有单一区带与洗脱峰对应；
On SDS-polyacrylamide gel electrophoresis (SDS-PAGE)the eluate from the column with the McAb coupled showed a single band, corresponding to the elution peak.
这使得定量洗脱四种分析物中的三种产生一个尖锐峰。
This allows quantitative elution of three of the four analytes as one sharp peak.
方法采用HPLC法，以君药红参中人参皂苷为检测指标，采用梯度洗脱的方法，控制其中红参主要成分的色谱峰。
Method The main ingredient of the injection, Red Ginseng, was determined by HPLC and used binary solvent-delivery gradient elution to control the main component in Red Ginseng.
重点讨论了流动相流速对分离度、峰容量和溶质洗脱顺序的影响。
It is mainly for the effect on the resolution, peak capacity and solute elution order.
方法：利用RPHPLC ，采用线性梯度洗脱等进行指纹图谱的测定，并应用LC/MS技术指认指纹图谱中的主要色谱峰。
Method: RP- HPLC, linear gradient elution, LC/MS, etc. were used to determine the fingerprint and identify the main pea ks in the HPLC fingerprint.
方法：采用梯度洗脱的方法进行色谱分离，对各色谱峰进行数据处理，得出红花黄色素的指纹图谱。
Methods: The gradient elution mode was applied in chromatographic separation and data were analyzed to develop HPLC fingerprint analysis of Safflor Yellow.
用初始流动相乙腈-水(40：6 0)洗脱至主峰出峰保留时间的2.3倍时开始进行梯度洗脱比较合理。
The gradient elution is reasonable when the retention time is 2.3 times after the appearance of prominent peak with initial mobile phase acetonitrile-water (40:60).
提问：色谱问题：USP如何处理在当前提议通则中出现色谱峰同时洗脱的问题？
Q2:Chromatographic question: How does USP propose to deal with peak co—elutions in the current proposed chapter?
结果表明，SCSP凝胶柱层析洗脱曲线为单一狭窄对称峰，说明为均一组分多糖；
The results show that SCSP gel column chromatography elution curve for a single narrow symmetrical peak, explain for homogeneous components polysaccharide;
每种组分有其特有的洗脱时间，仪器的响应值，峰面积和峰高都与组分的量有关。
The elution time is a characteristic of an individual compound; and the instrument response, measured as peak area or peak height, is a function of the amount present.
每种组分有其特有的洗脱时间，仪器的响应值，峰面积和峰高都与组分的量有关。
The elution time is a characteristic of an individual compound; and the instrument response, measured as peak area or peak height, is a function of the amount present.
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