目的建立不同组织来源成纤维细胞的培养方法并比较其培养差异。
Objective To set up the method of the culture of human fibroblasts from different sources and compare their discrepancy.
结论：胰酶消化、差速贴壁法是一种适合的心室成纤维细胞的培养方法。
Conclusion: Trypsin enzymic digestion and differential attachment was suitable for culture of ventricular fibroblast.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
不过，如同所有基因一样，在几乎任何细胞中都能找到它们的踪影，人们常常把特殊培养的成纤维细胞拿来研究，因为这样操作起来比较容易。
However, they are-like all other genes-found in almost all cells and are frequently studied in especially cultured fibroblast cells that are easy to work with.
目的探讨皮肤成纤维细胞在生物反应器中批式和间歇换液培养过程的生长、扩增与代谢特性。
Objective to investigate the growth, expansion, and metabolic characteristics of the human dermal fibroblasts cultured in a bioreactor with batch and medium exchange modes.
结果表明，体外培养人胚肺二倍体成纤维细胞成活至52代，而含0。2%黄芪制取液的培养基使细胞成活至77代。
The results showed that heldf cultured in vitro lived up to52generations while the culture medium containing0.2% amb extract made the cells live up to77genera-tions.
方法：采集健康的牙龈组织和牙周膜，体外培养牙龈成纤维细胞和牙周膜成纤维细胞。
Methods: Healthy human gingiva and periodontal ligament were acquired from patients, then gingival fibroblasts and periodontal cells were cultured in vitro.
结果(1)培养的细胞经形态学观察和波形蛋白sp染色，鉴定为成纤维细胞。
Results (1) the cultured cells identified by morphology and SP staining with vimentin showed the characteristic of fibroblasts.
分化培养中加入碱性成纤维细胞生长因子能够提高胆碱能神经元分化的比例。
The addition of basic fibroblast growth factor in differentiation culture could increase the proportion of cholinergic neurons.
目的和方法：利用细胞培养和RT P CR技术研究新生大鼠心脏成纤维细胞增加心肌细胞搏动频率的作用。
Aim and Methods: To study the effect of ventricular fibroblasts increase beating rate on cultured neonatal rat cardiomyocytes by using cell culture and RTPCR technique.
结论EGF对体外培养大鼠真皮成纤维细胞的增殖具有促进作用。
Conclusion EGF had the promotional effects on proliferation of the rat dermal fibroblasts in vitro.
这种多孔丝素膜具有良好的生物相容性，并可在多孔丝素膜中进行人表皮细胞的培养，毛细血管和成纤维细胞等能够长入其内而成活。
Due to the good biocompatibility of this membrane, the cuticle cell of human can be cultivated in it, also the capillary vessel and the fibroblast can grow in it.
目的：研究新生毛细血管对体外培养人皮肤成纤维细胞增殖的影响，探讨新生毛细血管在扩张过程中的作用。
AIM: To investigate the effects of new blood capillaries on the proliferation of dermal fibroblasts in vitro and the role of new capillaries during the period of dilatation.
目的探讨氦氖激光体外照射对培养的瘢痕成纤维细胞生长的抑制作用。
Objective to explore the inhibitory effect of He-Ne laser irradiation on growth of fibroblasts of hypertrophic scars in culture.
大鼠胚胎成纤维细胞(REF)可以作为培养大鼠胚胎干细胞的滋养层。
Rat embryonic fibroblasts (REF) can be used as feeder cells in cultivating rat embryonic stem cells.
以体外培养的布尔山羊和黄牛耳组织成纤维细胞为研究对象，进行了哺乳动物体细胞的冻存与冷藏试验。
The experiment of cryopreservation and cold storage of fibroblast cells cultured in vitro of Boer goat and yellow cattle′s ear skin was conducted.
结论氦-氖激光对培养瘢痕成纤维细胞生长的抑制效应与能量密度、照射次数有关。
Conclusion Inhibitory effect of He Ne laser irradiation on growth of scar fibroblasts is tightly associated with energy density and irradiation times.
本文研究了波尔山羊耳成纤维细胞的体外培养，包括分离纯化和生长特征，同时还研究了成纤维细胞的冷藏和冷冻保存。
The paper is mainly about the culture of Boer goat ear fibroblasts, including the isolation, purification and growth character, and we also studied the cold storage and cryopreservation.
目的观察软x射线照射对体外培养的成纤维细胞增殖的影响。
Objective To investigate the effects of soft X rays radiation on mouse fibroblast proliferation in vitro.
目的：探讨骨髓成纤维细胞条件培养液对体外巨核系细胞生成的促进作用。
Objective To investigate the effect of bone marrow fibroblasts conditioned media on the expansion of megakaryocytes in vitro.
研究新生大鼠心室成纤维细胞条件培养液促进心肌细胞肥大的作用。
Objectivel To study the effect of ventricular fibroblast promotion hypertrophy on cultured neonatal rat cardiomyocytes.
结果：培养的三种细胞均符合血管内皮细胞、血管平滑肌细胞及成纤维细胞的形态特征。
Results : the morphological characteristics of three kinds of cells are accord with those of vascular endothelial cells , smooth muscle cells and fibroblast.
结论：滤网过滤改良法可以在较短时间内培养出优质高采的鼠胚胎成纤维细胞。
CONCLUSION: Reformed filter method is proved to be the better method to isolate and culture MEF in short times.
目的：探讨激光对体外培养瘢痕成纤维细胞胶原合成的选择性抑制作用。
To study the effects of pulsed Nd: YAG laser irradiation on collagen production of scar fibroblasts.
结果表明，体外培养人胚肺二倍体成纤维细胞成活至52代，而含0.2%黄芪制取液的培养基使细胞成活至77代。
The results showed that HELDF cultured in vitro lived up to 52 generations while the culture medium containing 0.2% AMB extract made the cells live up to 77 generations.
方法：采用成纤维细胞体外培养技术，比较正常皮肤成纤维细胞与胎儿皮肤成纤维细胞对羊水的不同反应。
METHODS: a comparison of response to amniotic fluid was performed between the fibroblasts of the skins from fetus and from normal skin with the technique of in vitro culture.
目的观察人皮肤成纤维细胞在立体培养条件下的生长、增殖及代谢情况。
Objective To investigate the proliferation and metabolism of human dermal fibroblasts in fibrin gel-a three-dimensional cell culture system.
用不含血清和含0.5%血清的培养液直接饥饿指数生长期的成纤维细胞，细胞数量先是略有增长，然后逐渐下降。
After used medium in eluding 0.5% NCS and without NCS direct starvation, the Numbers of fibroblast first in creased slightly, then gradually decreased.
观察了真皮提取物对体外培养的人皮肤成纤维细胞生长的作用。
The author has observed that the extract from normal human dermis has obvious inhibitory effects on human skin cultured fibroblasts.
结果表明：胎鼠成纤维细胞MEF培养组的囊胚率和胚胎细胞数38。
The result showed that the mouse embryonic fibroblasts MEF group achieved significantly higher blastocyst formation rate 38.
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