表达产物在培养基中,表达的产量依赖于不同的基因序列、载体、宿主和诱导表达条件等因素。
The yield of gene product in culture medium depends on different gene sequences, vectors, hosts and i-uduced condition.
方法:采用PCR,克隆及连续亚克隆,序列分析,原核温度诱导表达等方法。
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
序列的构造方法有剪接法,升级法,选定法以及诱导法等。
M sequence has several constructive methods such as cut-joining, spanning tree, the selected method, the induced method and so on.
光诱导引起结构变化的特性现在可以专门用于综合控制细胞信号序列,如核输出信号(NES)。
The property of light-induced structure change can now be used specifically to synthetically control cellular signal sequences -- like the nuclear export signal (NES).
更特别的,本发明描述了赋予联结的核苷酸序列化学诱导而不是伤口或病原体诱导性表达的新启动子。
More specifically, this invention describes novel promoters that confer chemically inducible but not wound - or pathogen-inducible expression to an associated nucleotide sequence.
更特别的,本发明描述了赋予联结的核苷酸序列化学诱导而不是伤口或病原体诱导性表达的新启动子。
More specifically, this invention describes novel promoters that confer chemically inducible but not wound - or pathogen-inducible expression to an associated nucleotide sequence.
应用推荐