寡核苷酸探针的标记非常重要。
目的设计一种带有通用序列标签的寡核苷酸探针以应用于寡核苷酸芯片点样的质量控制。
Aim To design an universal sequence-tagged oligonucleotide probes for spot quality control of oligonucleotide microarrays.
结果:分析28种分支杆菌标准菌株和9种非分支杆菌菌株,结果显示寡核苷酸探针是特异的。
Results:The standard strains of 28 mycobacteria and 9 nonmycobacteria by oligonucleotide probe array were analyzed, which showed that the oligonucleotide probe was specific.
试验结果表明,设计的寡核苷酸探针具有一定的特异性,能很好的检测乳酸杆菌中SLP基因。
The result showed that the probe above could effectively detect the SLP gene with a certain specificity.
结果以HIV寡核苷酸探针作为核心序列,在其一端或两端连接上多种通用序列标签(UST)构建新型探针。
Results HIV oligo probes used as core target sequences were linked with several universal sequence tags (USTs) at their one or both ends, and were then printed onto the slides.
结果:SARS阳性样品与寡核苷酸芯片杂交后出现阳性杂交信号,具有不同二级结构的寡核苷酸探针杂交信号强度不一。
Results: Positive SARS samples were detected by oligonucleotide array and fluorescence signal intensity was highly related to the probe's second structure.
方法:利用合成的寡核苷酸探针对60例慢性牙周炎患者60患病位点、10例健康人的10个对照位点龈下菌斑中伴放线菌放线杆菌进行检测。
Methods: Samples of subgingival plaque were detected for Aa distribution by oligonucleotide probe from 60 sites of CP patients and 10 healthy sites of healthy people.
寡核苷酸固定过程中,末端氨基修饰没有明显的特异性,但是可以提高被固定探针的杂交容量。
During oligonucleotide immobilization, terminal amino modification has no obvious specificity, but it can enhance the hybridization capacity of immobilized oligonucleotides.
方法制备了包含87个心血管疾病相关基因探针的寡核苷酸芯片。
Methods Oligonucleotide microarrays consisting of 87 probes representing 87 human cardiovascular disease-related genes were constructed.
方法制备了包含87个心血管疾病相关基因探针的寡核苷酸芯片。
Methods Oligonucleotide microarrays consisting of 87 probes representing 87 human cardiovascular disease-related genes were constructed.
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