密度梯度离心法分离肝内淋巴细胞；
The intrahepatic lymphocytes from each group were isolated by using density gradient centrifugation.
采用蔗糖密度梯度离心法分离肝脏内质网。
Hepatic endoplasmic reticulum was isolated by differential centrifugation with sucrose density gradient.
密度梯度离心法体外培养骨髓间充质干细胞。
Methods Dental pulp stem cells were cultured in vitro, purified and then subcultured .
用改进的不连续蔗糖密度梯度离心法分离到五条带。
Five zones were separated by improved discontinuous sucrose gradient centrifugation.
方法不连续密度梯度离心法分离纯化大鼠肝贮脂细胞。
METHODS Rat lipocyte was isolated and purified with discontinuous density-gradient centrifugation.
骨髓单个核细胞取自同系健康大鼠的四肢长骨，用密度梯度离心法获得。
BM MNCs were isolated from long bone of limbs in healthy Lewis rats by centrifugation through a histopaque density gradient.
用密度梯度离心法分离并计数支气管肺泡灌洗液（BALF）中的嗜酸性粒细胞；
The eosinophils in broncho-alveolar lavage fluid (BALF) were separated by density gradient centrifugation.
目的比较免疫磁珠法和密度梯度离心法分离纯化人外周血中性粒细胞的可靠性。
Objective To compare the reliability of immunomagnetic beads and density gradient centrifugation in purification and isolation of neutrophilic granulocytes from human peripheral blood.
用密度梯度离心法分离后MEM培养基体外培养的人胚胎msc纯度高，生物活性好。
The purity of Human embryonic MSC which isolated by density gradient centrifugation and cultured by MEM culture medium in vitro is high. The activity of MSC is good.
目前国外采用较多的有荧光激活细胞分选法、磁活化细胞分选法、免疫磁珠分离法、密度梯度离心法。
Various methods of enrichment exit:fluorescent activated cell sorting, magnetic-activated cell sorting, immunomagnetic beads, density gradient.
方法：抽取兔髂骨及胫骨的骨髓液，采用密度梯度离心法结合贴壁培养法分离纯化出MSC，并增殖。
Methods: MSC were aspirated from rabbit iliac and tibias crest and purified by gradient centrifuge and adhesive culture methods.
方法：抽取兔髂骨及胫骨的骨髓液，经密度梯度离心法及塑料板贴壁培养法分离纯化出msc，并增殖。
Methods: MSC were harvested from iliac and tibias crest and obtained by using gradient centrifuge and adhesive culture methods.
结论采用全骨髓直接培养及密度梯度离心法，经3 ~5代培养后均可获得相对纯化之骨髓间充质干细胞。
Conclusion Byuse complete marrow direct culture method and density gradient method, after 3 ~ 5 passages, relative purified BMSCs can be obtained.
将腮腺炎病毒S79株培养液用中空纤维超滤器进行浓缩，经PEG沉淀后，采用蔗糖密度梯度离心法纯化抗原；
The tissue culture of mumps virus S79 strain was purified by ultrafiltration on hollow fiber, PEG precipitation and density gradient ultracentrifugation.
方法：密度梯度离心法分离44例CHF患者和20例正常人PBMC，酶消化和差速贴壁法分离乳鼠心肌细胞。
Meathod: The experiment adopted density gradient centrifuging to separate PBMC of 44 patients with CHF and 20 normal person and enzyme digestion to separate neonatal rat cardiac myocyte.
方法：取健康新西兰幼兔骨髓，密度梯度离心法分离骨髓间充质干细胞并进行体外扩增培养、成骨诱导分化及鉴定。
METHODS: Bone marrow was obtained from healthy New Zealand rabbits, by using density gradient centrifugation BMSCs were isolated and were then cultured in vitro.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
方法使用密度梯度离心法分离不同年龄段脂肪间充质干细胞进行培养，保留贴壁细胞传代，分析脂肪间充质干细胞的纯度，观察细胞生长情况，检测其增殖活性、细胞周期。
The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
应用推荐