伴有辅酶再生的多酶反应技术是酶工程领域的重要课题。
One of the important topics in the enzyme engineering is the multienzyme reaction systems coupled with the coenzyme regeneration.
酶的分离、纯化、固定化等技术的研究，以及多酶反应器的研制等。
The enzyme purified, separated and immobilized and also the reactors for enzyme, develope…
每个实验室都有一台实时多聚酶链式反应机，有一个实验室有两台这样的机器。
Each lab now has a real-time polymerase chain reaction (PCR) machine, and one lab has two machines.
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
结果表明：在化学反应体系中，菜籽多酚级分- 2具有还原能力，能清除活性氧，抑制脂肪氧合酶的活性。
The results showed that RSPP-2 is a good deoxidant and can scavenge reactive oxygen species and inhibit lipoxygenase in some modified chemical systems.
实验发现L-半胱氨酸对该酶有明显的抑制作用，它的作用可以导致马铃薯多酚氧化酶的酶促反应的迟滞时间延长，同时使稳态酶活力下降。
The existence of L-Cysteine resulted in prolonging the lag time of the enzyme reaction, while the steady-state rate of the enzyme activity decreased with the increase of concentration of L-Cysteine.
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
一种定量实时多聚酶链反应能够快速准确的诊断巨细胞病毒，改善免疫抑制患者的治疗。
A quantitative real-time polymerase chain reaction test provides rapid and accurate diagnosis of cytomegalovirus and improved management of immunocompromised patients.
结果：所有病例都成功地进行了DNA提取和多聚酶链反应扩增。
Results: DNA extraction and polymerase chain reaction amplification was successful in all cases.
结果表明，固定床反应器的酶吸附比釜式多，而酶的活性变化在两种型式反应器中比较接近。
The results showed amount of enzyme adsorption were more in fixed bed reactor than in tank reactor, and there were close variations of enzyme activity in two types of reactor.
方法应用多聚酶链反应技术及限制性片段长度多态现象对46例食管癌APC和MCC基因的LOH进行了分析。
Methods LOH at APC and MCC genetic loci in 46 specimens resected from esophageal neoplasm was studied with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
逆转录多聚酶链反应检测间质胶原酶及金属蛋白酶组织抑制因子- 1基因表达水平。
The gene expression levels for interstitial collagenase and tissue inhibitor of metallo-proteinase-1 (TIMP-1) were measured by reverse transcription polymerase chain reaction.
方法采用多对型特异性引物-聚合酶链反应检测160例慢性乙型肝炎患者血清HBV基因型；
Methods: Serum samples from 160 cases with chronic HBV infection were collected and tested for HBV genotypes by type-specific primers.
方法应用逆转录酶-多聚酶链反应(RT - PCR)方法，检测了83例食管癌和贲门癌组织及28例患者45枚淋巴结中MRP基因的表达，并与相应癌旁组织进行对照分析。
Methods RT-PCR was applied to study expression of MRP gene in tumor tissues from 83 cases of esophageal and cardiac carcinoma, and 45 lymph nodes from 28 patients.
我们相信用更灵敏的多聚酶链式反应(PCR)技术检测胃脱落细胞，对患癌危险性的早期预报会有很大帮助。
We believe that, with the more sensitive method of Polymerase Chain Reaction (PCR), we can test the fallen cells of stomach hoping that it would be helpful to the early diagnosis of stomach cancer.
方法：应用多聚合酶链反应技术(PCRT)，对10份遗传性卵巢癌组织中BRCA1基因内部的D17S855微卫星位点进行LOH检测。
Methods: Using the polymerase chain reaction technique (PCRT), LOHs in 10 samples of hereditary ovarian cancer at intragenic loci were detected.
多聚酶链反应(PCR)具有灵敏度高，特异性强，快速高效的特点，在病原微生物检测领域有广阔的前景。
Polymerase chain reaction (PCR) has many advantages, such as high sensitivity, strong specificity and high-speed. There are vast vistas in the examination of pathogenic microorganism.
所用的DNA方法学和所处的妊娠期对检测精度有着最大的影响，实时定量多聚酶链反应(RTQ -PCR)的检测精度超过常规的PCR。
DNA methodology and gestational age had the largest effects on test performance, with real-time quantitative polymerase chain reaction (RTQ-PCR) outperforming conventional PCR.
我们将人脑动脉瘤和动静脉血管畸形中的内皮细胞分离出来，并用内皮标志物结合多聚酶链反应和免疫组化等方法确认其内皮来源性。
We isolated ECs from human AVM and aneurysm and then confirmed their EC origin by polymerase chain reaction and immunocytochemistry with endothelial markers.
方法：通过半巢式逆转录多聚酶链反应检测HGV。
结论：滤纸条取液技术取样作沙眼衣原体的多聚酶链反应检测效果好。
The positive rates between different types of tubal pathological changes are not obviously different. Conclusions: The filter paper technique is an effective method in detecting CT-DNA by PCR.
采用多聚酶链反应-限制性片段长度多态性法(PCR - RFLP)分析MGP和ALAD基因的多态性。
The polymorphisms of MGP gene and ALAD gene were analyzed by the methods of PCR-RFLP.
方法：运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR -RFLP)检测MTHFR的677位点多态性。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
方法：运用多聚酶链反应-限制性内切酶片段长度多态性技术(PCR -RFLP)检测MTHFR的677位点多态性。
Methods: PCR-RFLP technique was used for detecting the A677V polymorphism site of MTHFR gene.
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