由于这一增长,我们部署涵盖数百数千以前机器生成的检测多态性检测。
As this grows, we deploy polymorphic detections which cover hundreds to thousands of previously machine generated detections.
当前的基因检测分析了大约600,000个SNP(单核苷酸多态性),只是整个基因组的千分之一。
Current genetic tests analyze about 600,000 SNPs (single nucleotide polymorphisms) or 0.1 percent of the entire genome.
基因多态性研究可为预防adr提供新的参考依据,临床可在药物治疗前对患者进行基因型检测,以降低adr的发生率。
Gene polymorphism study can provide a new reference for the prevention of ADR. Gene detection can be conducted before drug treatment to reduce the incidence of ADR.
本研究也证明了RAPD和SSR两种分子标记在检测不同的AA组型水稻品种间多态性方面是很有效的方法。
It is also demonstrated from this study that both RAPD and SSR analyses are powerful methods for detecting polymorphisms among the different AA genome Oryza accessions.
首先从实验对象的外周血中提取DNA,pcr扩增il-4的第二个内含子中的一段TG重复序列,然后用8%的聚丙烯酰胺凝胶电泳检测这段基因的多态性。
DNA were extracted from the peripheral blood, then TG sequence in second intron of IL - 4 was amplified by PCR, the polymorphism of that segment were then detected through 8% polyacrylamide gel.
以BMPR - IB基因作为候选基因,采用PCR -RFLP方法检测其在萨福克羊、小尾寒羊及萨寒f 1代杂交羊中的多态性分布,分析其与产羔数的相关性。
With BMPR-IB gene as candidate gene, its polymorphism distribution in Suffolk sheep, small-tail Han sheep and their F1 hybrid germination were detected by using the method of PCR-RFLP.
方法:采用PCR及微卫星多态性技术,对65例肝癌1号染色体上28个微卫星标志位点杂合性缺失进行检测。
Methods: LoH of chromosome 1 were detected by PCR based microsatellite polymorphism analysis technique using 28 pairs of microsatellite markers primers in 65 HCC.
扩增片段长度多态性(AFLP)被称为“下一代分子标记”,是检测DNA多态性的一种新技术。
Amplified Fragment Length polymorphism (AFLP) is called a "descendant molecular marker", and it is a new technique in detecting DNA polymorphism.
本文建立了一个基于连接酶检测反应的基因多态性并行检测系统,该系统应用连接酶检测反应与基因芯片技术。
Typing technology is the main method to evaluate gene polymorphism. The parallel typing system based on ligase detection reaction is (established) for gene polymorphism.
用单链构象多态性对扩增片段进行点突变检测。
The point mutations in these fragments were detected by SSCP.
本研究采用淀粉凝胶电泳法对我国长江流域五个沼泽型类群的395头水牛型清白蛋白多态性进行了检测。
The serum albumin polymorphism of 395 animals from five swamp buffalo types in the Changjiang River valley were analyzed by starch gel electrophoresis.
目的:评价异源双链分析(HET)和单链构象多态性分析(SSCP)在基因突变检测中的作用。
Objective:To assess the roles of single stranded conformation polymorphism(SSCP)and heteroduplex polymorphism(HET)in screening gene mutation.
对单亲案的亲权鉴定,检测的多态性基因座要在8个以上。
For the purpose of identifying paternity in cases of absence of mother, more than 8 polymorphic DNA loci must be tested.
利用所筛选的多态性标记对BC_1F_1群体进行前景检测,检测到161个供体片段,并对含有供体片段的单株进行背景选择和回交。
Doing foreground selection of BC_1F_1 with polymorphic locus, 161 donor segment were detected, and doing background selection and backcross for plants containing donor segment.
采用等电聚焦电泳对中国矮马进行了血红蛋白多态性的检测。
Haemoglobin polymorphism of Chinese miniature horses was examined by agarose gel isoelectric focusing.
结果:荧光定量方法可以快速完成MTHFR C677T基因多态性的检测,检测结果得到传统PCR-RFLP方法的证实。
Results : Detection of MTHFR gene C677T polymorphism was quickly finished by realtime fluorescence quantitative RT - PCR, and the results were proved to be effective by PCR - RFLP.
本文首次运用MSAP技术对脐橙品种进行胞嘧啶甲基化分析表明,使用MSAP检测脐橙品种多态性非常有效。
This paper is the first time to analyse cytosine methylation in navel oranges using MSAP, indicating MSAP technique is efficient for detection of cytosine methylation in navel orange genomes.
方法应用聚合酶链反应限制性片段长度多态性分析方法(PCR -RFLP)对40例口腔鳞癌组织中apc基因的杂合缺失(LOH)进行检测。
Methods We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to examine 40 oral squamous cell carcinomas for loss of heterozygosity (LOH) at APC.
目的:(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBVDNA阳性的上海籍HBV感染者的基因型。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
这31条多态性引物和所检测到的93个多态性位点初步为草鱼基因组的多态性分析提供了可靠的分析指标体系。
Both the 93 polymorphic loci and the 31 polymorphic primers might provide a reliable molecular marker system for grass carp genomic DNA polymorphism analysis.
酚-氯仿法从外周血中提取基因组dna,聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
SNP位点是目前基因多态性研究的主要内容,包括检测分型和功能活性研究两个层次,已经建立了高度自动化和高通量的SNP检测分型技术。
Single Nucleotide polymorphism (SNP) site is the main content of genetic polymorphism study, which includes detection of gene type and research on function and activity.
方法:采用PCR技术和限制性内切酶片段长度多态性(RFLP)的方法,检测中国北方汉族168个核心家系的基因型。
Methods A PCR-based RFLP procedure was employed to detect the genotypes of 168 family trios of Han descent population in the North of China.
目的:检测青海土族和撒拉族健康个体HLADQA1和DQB1等位基因频率,了解和分析这两个民族的HLA DQA1和DQB1基因多态性。
Objective:To clerify HLA DQA1 and DQB1 gene polymorphisms in a population of Tu and Sala nationalities from two areas in Qinghai province.
结论DNA多态性分析技术可快速、准确地检测患者移植物的植入状态,为临床移植提供准确、可靠的实验指标,为进一步制定治疗方案提供依据。
Conclusion DNA polymorphism analysis technique can detect engraftment status of patients exactly, rapidly, which provides effective evidences of constitution for more clinical therapy projects.
该文讨论了适用于常规实验室的一些单核苷酸多态性(SNP)检测方法,比较了不同方法在SNP检测中的优缺点,并介绍了一些新的方法。
Some SNP assay methods, including newest methods, adopted in common laboratory were introduced in this article. Their advantages and disadvantages were discussed.
方法:应用免疫组织化学和聚合酶链反应—单链构象多态性分析(PCR-SSCP)检测10例舌白斑和32例舌癌,并结合临床资料进行分析。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
方法:应用免疫组织化学和聚合酶链反应—单链构象多态性分析(PCR-SSCP)检测10例舌白斑和32例舌癌,并结合临床资料进行分析。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
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