本文综述了发根农杆菌诱导植物毛状根的方法及其分子机制。
The method of hairy root induction of plants as well as their molecular biology are reviewed.
本文研究了茶叶儿茶素对发根农杆菌的抑制作用并进行了抗酚菌株的筛选。
This paper investigated inhibition of Agrobacterium rhizogenes by tea catechin and screening of the anti-polyphenol strain.
方法分别用A4、LBA9402和C58C1三种发根农杆菌菌株转化莨菪子叶受体，获得发根；
Methods Hairy root of H. niger was obtained from cotyledon explants after infected with Agrobacterium rhizogenes strains A4, LBA9402, and C58C1, respectively.
结果：高压纸电泳结果显示，所获毛状根中均含有甘露碱，表明毛状根中确已转入发根农杆菌的T-DNA；
Results: The results of paper electrophoresis showed that the hairy roots contained mannopine, this evidenced the integration of Ri T DNA in the transformed plants;
用发根农杆菌(agrobacteriumrhizogenes)的ri质粒转化药用植物牛膝(achyranthes bidentataBl。)，诱导出了抗卡那霉素的发状根。
Achyranthes bidentata BL, a famous medica plant, was transferred by ri plasmid with Agrobacterium rhizogenes, and hairy root with kanamycin resistance was induced successfully.
用发根农杆菌(agrobacteriumrhizogenes)的ri质粒转化药用植物牛膝(achyranthes bidentataBl。)，诱导出了抗卡那霉素的发状根。
Achyranthes bidentata BL, a famous medica plant, was transferred by ri plasmid with Agrobacterium rhizogenes, and hairy root with kanamycin resistance was induced successfully.
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