方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
对40份盲样采用琼脂扩散试验(agp)、血凝抑制试验(HI)和反转录聚合酶链反应(RT - PCR)进行了检测。
40 blind samples were examined by AGAR gel precipitation (AGP), hemagglutination inhibitory (hi) test and reverse transcription-polymerase chain reaction (RT-PCR).
采用反转录-聚合酶链反应检测两组大鼠大网膜和皮下脂肪组织中抵抗素基因的表达。
The RT-PCR was adopted to detect the expressions of resistin gene in epiploon and subcutaneous fatty tissues.
半定量反转录-聚合酶链反应验证了芯片分析结果的可靠性。
The results of semi-quantitative RT-PCR experiments supported the reliability of our microarray analysis.
反转录-聚合酶链反应法检测CXCR4的表达，亦得到相似结果。
Similar results were obtained through detecting the expression of CXCR4 with reverse transcription-polymerase chain reaction.
反转录-聚合酶链反应法检测CXCR4的表达，亦得到相似结果。
Similar results were obtained through detecting the expression of CXCR4 with reverse transcription-polymerase chain reaction.
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