同时有轻微的去甲基化反应发生。
组蛋白赖氨酸去甲基化酶有可能成为一个新的抗肿瘤治疗靶标。
Therefore, histone lysine demethylases may serve as a new target for anti-cancer therapy.
本研究发现从全序列水平来看，水稻胚乳DNA是去甲基化的。
Here, we show that rice endosperm DNA is hypomethylated in all sequence contexts.
目的探讨胃癌组织中甲基化酶、去甲基化酶基因与肿瘤相关癌基因和抑癌基因的关系。
Objective To investigate the relationship of the expression of DNA methyltransferase, demethylase(mbd2) and tumor-associated genes in human gastric cancer.
随着组蛋白赖氨酸去甲基化酶的发现，证实组蛋白赖氨酸甲基化是一个可以逆转的组蛋白表遗传修饰。
The discovery of the histone lysine demethylases has strongly demonstrated that histone lysine methylation is a reversible epigenetic modification.
本发明属于植物基因工程技术领域，具体涉及一种水稻组蛋白去甲基化酶基因及其编码蛋白与应用。
The invention belongs to the technical field of plant gene engineering, and more particularly relates to a paddy rice histone demethyl methylase gene, coded protein and the application thereof.
氮-2'-脱氧胞苷等去甲基化药物作为抗肿瘤药物的效应，归因于两种机制：细胞毒性和诱导低甲基化。
The efficacy of hypomethylating agents (e. g. 5 aza 2′ deoxycytidine) as antineoplastic agents has been attributed to two distinct mechanism: cytotoxicity and induction of hypomethylation.
DNA甲基化变化频率递减的顺序依次是：V27、V14、R 09； V27品系显现出更多的去甲基化的位点。
Frequencies of decrease in DNA methylation sites among cultivars were in this order: V27, V14, and R09, V27 showing more demethylation sites.
据此，人们开发出了去甲基化药物，并已应用于临床治疗及研究，结果证实去甲基化药物是一类安全、有效的抗肿瘤新药。
Accordingly, the demethylation drugs were developed and they have been used in clinical treatment and research, which confirmed that the drug is a kind of safe and effective anti-tumor drug.
结论EGCG能使RASSF1A基因去甲基化，并上调其非甲基化基因和蛋白的表达水平，这可能是其抗肿瘤的重要机制。
Conclusion Demethylation of RASSF1A and increasing its mRNA and protein induced by EGCG may be one of the mechanisms of its antineoplastic activity.
SDS - PAGE、圆二色谱和MALDI - TOF质谱分析结果表明，成功获得纯度较高的重组蛋白，且具有去甲基化的活性。
Results of SDS-PAGE, CD and MALDI-TOF showed that the recombinant protein was obtained successfully with high purity and possessed demethylation activity.
而在2 -4小时期间，甲基化水平迅速上升，并超过去甲基化水平，在4 - 16小时期间甲基化水平趋于稳定，并且始终高于去甲基化水平。
In the 2-4 hour, the increasing speed of methylate shoot up and exceeded the level of demethylation. In the 4-16 hour, the level of methylation went to stable and kept on the higher level.
靶向DNA甲基化和组蛋白去乙酰化酶的几个表观药物已经在临床试验中被测试。
Several epigenetic drugs targeting DNA methylation and histone deacetylation enzymes have been tested in clinical trials.
它依赖于用亚硫酸氢钠处理后，非甲基化的 胞嘧啶选择性的去氨基为 尿嘧啶，通常接着所选的靶向区域的PCR扩增。
It relies upon the selective deamination of unmethylated cytosine to uracil after treatment with sodium bisulphite, usually followed by PCR amplification of the chosen target region.
它依赖于用亚硫酸氢钠处理后，非甲基化的 胞嘧啶选择性的去氨基为 尿嘧啶，通常接着所选的靶向区域的PCR扩增。
It relies upon the selective deamination of unmethylated cytosine to uracil after treatment with sodium bisulphite, usually followed by PCR amplification of the chosen target region.
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