比较几丁质酶处理前后标本的外部形态。
Morphology of the samples treated with or without the chitinase were compared.
几丁质酶是植物抗真菌基因工程的热点之一。
Chitinases are one of hot points in plant genetic engineers of fungal resistance.
着重论述了提高微生物产几丁质酶能力的方法。
The emphasis was laid on the review of production capability of chitinase by microbes.
本文叙述了植物几丁质酶的特性、结构和功能、基因结构；
This review summed up the characters, structure and function of plant chitinases and their genes' structure;
利用基因枪法，以菜豆几丁质酶基因转化小麦幼胚愈伤组织。
Using embryogenic calli from young embryos as explants, bean chitinase gene was transferred into spring wheat plants by microprojectile bombardment.
考察八种碳源对苏云金杆菌HD224菌产几丁质酶的影响。
The synthesis of chitinase by Bacillus thuringiensis HD224 strain using eight different carbohydrate resources was studied.
几丁质酶是高等植物普遍存在的一种与抗真菌病害有关的酶。
Chitinases are a kind of enzymes related to anti fungal disease, which universally exist in higher plant.
潮霉素抗性浓度的筛选为检测谷子转几丁质酶基因奠定基础。
The resistance concentration of hygromycin selected is the basis for testing transgenes millet plant.
昆虫几丁质酶是分解昆虫体壁和中肠围食膜几丁质的重要酶类。
Insect chitinases are a group of important chitinolytic enzymes, which hydrolyse the cuticle and peritrophic membranes of insects.
几丁质酶和葡聚糖酶在植物病害生物防治作用中具有重要的作用。
Chitinase and glucanase are very important in plant disease biological control.
几丁质酶1既是外切酶又是内切酶，而几丁质酶2只表现内切酶活力。
Chitinase 1 showed mixed activities of endo-and exo-chitinase, while chitinase 2 was an endo-chitinase.
PH梯度实验显示该几丁质酶在PH7.0环境条件下活性和稳定性最高。
The gradient test of PH showed that the chitinase activation and stablition were highest in PH7.0.
该几丁质酶完全不水解纯的片状几丁质，脱矿几丁质也不是该酶的良好底物；
The enzyme showed no activity on purified flaked chitin, little activity on de-mineralized chitin.
结果表明植物源的抗菌肽基因与几丁质酶基因在抗植物真菌病害中具有协同增效作用。
The results suggested that there was a synergetic effect in plant antimicrobial peptide gene and chitinase gene against pathogenic diseases.
由此可以认为根虫瘟霉分泌几丁质酶活的变化与菌株侵染力的变化有一定的内在联系。
It is suggested that there were inherence relationship between chitinase activities excreted by Z. radicans isolates and their infectivity against P. xylostella.
人几丁质酶，其重组生产，其分解几丁质的用途及其在治疗和预防感染性疾病中的应用。
Human chitinase, its recombinant production, its use for decomposing chitin, and its use in therapy or prophylaxis against infection diseases.
经几丁质酶处理后，螨的外部形态保存完整，与处理前无明显差别，可以用于形态鉴定。
The morphology of oribatid mites treated with the chitinase has no obvious changes compared to the untreated ones, and could be used in the morphology species identification.
利用PDS10 0 0 /氦气基因枪将水稻几丁质酶基因导入小麦幼胚盾片愈伤组织。
Rice chitinase gene was transferred into immature embryogenic calli of wheat using PDS1000/He particle delivery system.
割胶树皮伤口的变化，并测定了乳管中重要的防卫蛋白质几丁质酶在树皮伤口的积累和消失。
By assaying the activities of the cut, chitinase, an important defense protein in the laticifer, was traced after bark wounding.
昆虫的几丁质防治昆虫是一种有效的途径，所以近年来几丁质酶的研究越来越受到研究者的关注。
Targeting on chitin in insect body, chitinase are taken more and more attention by researchers because it can degrade chitin then the objective of controlling pests can be achieved.
而稻瘟病菌几丁质酶表达的差异是否与菌株间致病性等生物学和生理学差异有关，有待进一步研究。
Whether the expression levers of the Chitinase is related to the differences of biological and physiological aspects such as pathogenicity among different strains still remains unclear.
比较了虾几丁质酶和木瓜溶菌酶对再生几丁质与溶壁微球菌作底物时的水解速度，证明各有其最适底物。
Two enzymes having the different hydrolytic products with regenerated chitin as substrate that show that the chitinase is an endo enzyme and the papaya lysozyme is an exo enzyme.
因此，开展新型苏云金芽孢杆菌资源的分离筛选、鉴定、分子生物学以及产几丁质酶相关条件的研究都具有重要意义。
Therefore, to study on the screening, identification, molecular biology and chitinase production of Bt is of great significance to discover new strains.
几丁质是昆虫外壳和围食膜的重要组成成分，在适当的时期昆虫分泌适量的几丁质酶降解几丁质以保证昆虫的正常生长。
Chitin is an important polysaccharide that occurs in the exoskeleton and gut linings of insects, and it was degraded by chitinase at appropriate time for the proper development stage of the organism.
结果表明 ：几丁质酶对镰刀菌引起的西瓜枯萎病有一定的抑制作用 ，利用花粉管通道法直接导入西瓜活体植株的技术是可行的。
The results indicated that the chitinase had certain inhibition effect on Fusarium oxysporum, and the method of pollen tube pathway could be successfuly used in watermelon transformation.
病原物的侵染诱导植物几丁质酶活性升高和产生新的几丁质酶同工酶，这些高活性的几丁质酶或特异性的同工酶提高了植物的抗病性；
The pathogens infection induced the chitinase activity increase and the chitinase isozymes change. The high chitinase activity or specific chitinase isozymes have enhanced disease resistance of plant.
对线虫有强致死作用的菌株并不具有较高的几丁质酶活，细菌对北方根结线虫二龄幼虫的致死作用与其分泌的几丁质酶活性的高低相关性不大。
The activities of chitinase for effective and non-effective strains had not much difference, and the strains which were strongly lethal to the juveniles did not have high activity of chitinase.
采用人工接种和酶活性测定的方法，研究转入几丁质酶基因等1～4个抗真菌基因的13个转基因水稻株系对纹枯病的抗性和几丁质酶活性变化规律。
The research was conducted to reveal the regular of resistance to sheath blight and chitinase activities of 13 transgenic rice lines which modified by chitinase gene and other anti-fungal genes.
主要对几丁质酶的特性、功能及其在植物真菌病害防治中的应用进行了综合论述。在此基础上，对几丁质酶在植物病害生物防治中的应用前景进行了展望。
In this paper, characteristic, function and prevent plant diseases of chitinase were summarized. On this basis, the prospects of chitinase applications in biocontrol of plant disease were promised.
对部分经潮霉素筛选得到的再生植株进行了多次重复P CR检测，发现其中40 %以上的潮霉素抗性植株均表现出较强的阳性反应，初步证明几丁质酶基因已整合到油菜细胞核基因组中。
PCR test of the resistant plants indicated that 40% of the Hyg-resistant plants showed strong positive reaction, suggesting that chitinase gene had been integrated into the genome of rapeseed.
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