目的:探讨改良的凝胶阻滞法用于核蛋白-DNA结合的检测。
To improve a gel retardation Assay for the study of binding in the nuclear protein and DNA.
表达产物用亲和层析一步法进行纯化,然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
卵清蛋白从海藻酸凝胶颗粒中释放的试验结果表明,由于上述海藻酸锌凝胶的特性,导致海藻酸锌对卵清蛋白扩散阻滞作用相对较强;
The result of release (experiments) of ovalbumin from alginate beads demonstrated that the diffusion of protein was slower in zinc alginate beads due to above characteristic of zinc alginate gels.
目的采用凝胶迁移阻滞法分析研究人类端粒酶dna结合特性。
Objective The specific property for DNA-binding of human telomerase was tested by gel mobility shift assay.
目的采用凝胶迁移阻滞法分析研究人类端粒酶dna结合特性。
Objective The specific property for DNA-binding of human telomerase was tested by gel mobility shift assay.
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